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Low Seroprevalence of SARS-CoV-2 Antibodies during Systematic Antibody Screening and Serum Responses in Patients after COVID-19 in a German Transplant Center.
Choi, Mira; Bachmann, Friederike; Naik, Marcel Ganesh; Duettmann, Wiebke; Duerr, Michael; Zukunft, Bianca; Schwarz, Tatjana; Corman, Victor Max; Liefeldt, Lutz; Budde, Klemens; Halleck, Fabian.
  • Choi M; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Bachmann F; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Naik MG; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Duettmann W; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Duerr M; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Zukunft B; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Schwarz T; Institute of Virology, Charité-Universitätsmedizin Berlin, German Center for Infection Research (DZIF), and Berlin Institute of Health, 10117 Berlin, Germany.
  • Corman VM; Institute of Virology, Charité-Universitätsmedizin Berlin, German Center for Infection Research (DZIF), and Berlin Institute of Health, 10117 Berlin, Germany.
  • Liefeldt L; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Budde K; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
  • Halleck F; Department of Nephrology and Medical Intensive Care, Charité- Universitätsmedizin Berlin, 13353 Berlin, Germany.
J Clin Med ; 9(11)2020 Oct 23.
Article in English | MEDLINE | ID: covidwho-895373
Semantic information from SemMedBD (by NLM)
1. Antibodies PART_OF 2019 novel coronavirus
Subject
Antibodies
Predicate
PART_OF
Object
2019 novel coronavirus
2. Antibodies TREATS Patients
Subject
Antibodies
Predicate
TREATS
Object
Patients
3. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
4. COVID-19 OCCURS_IN Kidney Transplant Recipient
Subject
COVID-19
Predicate
OCCURS_IN
Object
Kidney Transplant Recipient
5. COVID-19 PROCESS_OF Kidney Transplant Recipient
Subject
COVID-19
Predicate
PROCESS_OF
Object
Kidney Transplant Recipient
6. Enzyme-Linked Immunosorbent Assay USES immunoglobulin A
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
immunoglobulin A
7. Pharyngeal structure LOCATION_OF Nasal Swabs
Subject
Pharyngeal structure
Predicate
LOCATION_OF
Object
Nasal Swabs
8. Fluorescent Antibody Technique USES Vitronecti
Subject
Fluorescent Antibody Technique
Predicate
USES
Object
Vitronecti
9. Specimen Type - Serum PART_OF Outpatients
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Outpatients
10. Kidney Transplantation TREATS Recipient
Subject
Kidney Transplantation
Predicate
TREATS
Object
Recipient
11. Kidney Transplant Recipient LOCATION_OF Specimen Type - Serum
Subject
Kidney Transplant Recipient
Predicate
LOCATION_OF
Object
Specimen Type - Serum
12. Asymptomatic PROCESS_OF Patients
Subject
Asymptomatic
Predicate
PROCESS_OF
Object
Patients
13. Antibodies PART_OF 2019 novel coronavirus
Subject
Antibodies
Predicate
PART_OF
Object
2019 novel coronavirus
14. Antibodies TREATS Patients
Subject
Antibodies
Predicate
TREATS
Object
Patients
15. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
16. COVID-19 OCCURS_IN Kidney Transplant Recipient
Subject
COVID-19
Predicate
OCCURS_IN
Object
Kidney Transplant Recipient
17. COVID-19 PROCESS_OF Kidney Transplant Recipient
Subject
COVID-19
Predicate
PROCESS_OF
Object
Kidney Transplant Recipient
18. Enzyme-Linked Immunosorbent Assay USES immunoglobulin A
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
immunoglobulin A
19. Pharyngeal structure LOCATION_OF Nasal Swabs
Subject
Pharyngeal structure
Predicate
LOCATION_OF
Object
Nasal Swabs
20. Fluorescent Antibody Technique USES Vitronectin, human
Subject
Fluorescent Antibody Technique
Predicate
USES
Object
Vitronectin, human
21. Specimen Type - Serum PART_OF Outpatients
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Outpatients
22. Kidney Transplantation TREATS Recipient
Subject
Kidney Transplantation
Predicate
TREATS
Object
Recipient
23. Kidney Transplant Recipient LOCATION_OF Specimen Type - Serum
Subject
Kidney Transplant Recipient
Predicate
LOCATION_OF
Object
Specimen Type - Serum
24. Asymptomatic PROCESS_OF Patients
Subject
Asymptomatic
Predicate
PROCESS_OF
Object
Patients
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 denotes a global health issue. Data regarding COVID-19 incidence in kidney transplant recipients (KTR) are sparse. From 19 March to 19 May 2020, we performed a systematic screening for COVID-19 in KTR. Tests included serum analysis for SARS-CoV-2 antibodies using S protein-based immunofluorescence, anti-SARS-CoV-2 S1 immunoglobulin G (IgG) and immunoglobulin A (IgA) enzyme-linked immunosorbent assays (ELISA), and/or quantitative reverse transcription polymerase chain reaction (qRT-PCR) from nasal-throat swabs. Outpatient serum samples from KTR with PCR confirmed COVID-19, and swab samples from recipients (+donors) undergoing kidney transplantation were analyzed. Out of 223 samples from outpatients, 13 patients were positive with solely anti-SARS-CoV-2-IgA and 3 with both anti-IgA and anti-IgG. In total, 53 patients were symptomatic in the past, but positive results could be found in both symptomatic and asymptomatic patients. After an in depth analysis using immunofluorescence and neutralization tests in 2 KTR, recent COVID-19 infection remained highly suspicious. Apart from outpatient visits, only 5 out of 2044 KTR were symptomatic and tested positive via PCR, of which 4 recovered and one died. All patients showed seroconversion during the course of the disease. This study demonstrated a low seroprevalence in a German KTR cohort, and seroconversion of IgA and IgG after COVID-19 could be demonstrated. Effective containment strategies enabled us to continue our transplant program.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Screening study Language: English Year: 2020 Document Type: Article Affiliation country: Jcm9113401

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Screening study Language: English Year: 2020 Document Type: Article Affiliation country: Jcm9113401