Your browser doesn't support javascript.
Quantitative analysis of SARS-CoV-2 RNA from wastewater solids in communities with low COVID-19 incidence and prevalence.
D'Aoust, Patrick M; Mercier, Elisabeth; Montpetit, Danika; Jia, Jian-Jun; Alexandrov, Ilya; Neault, Nafisa; Baig, Aiman Tariq; Mayne, Janice; Zhang, Xu; Alain, Tommy; Langlois, Marc-André; Servos, Mark R; MacKenzie, Malcolm; Figeys, Daniel; MacKenzie, Alex E; Graber, Tyson E; Delatolla, Robert.
  • D'Aoust PM; Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada.
  • Mercier E; Department of Chemical Engineering, University of Ottawa, K1N 6N5, Canada.
  • Montpetit D; Department of Chemical Engineering, University of Ottawa, K1N 6N5, Canada.
  • Jia JJ; Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada.
  • Alexandrov I; ActivSignal LLC., 27 Strathmore Rd Natick, MA 01760, United States.
  • Neault N; Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada.
  • Baig AT; Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada.
  • Mayne J; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa K1H 8M5, Canada.
  • Zhang X; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa K1H 8M5, Canada.
  • Alain T; Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa K1H 8M5, Canada.
  • Langlois MA; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa K1H 8M5, Canada.
  • Servos MR; Department of Biology, University of Waterloo, Waterloo N2L 3G1, Canada.
  • MacKenzie M; ActivSignal LLC., 27 Strathmore Rd Natick, MA 01760, United States.
  • Figeys D; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa K1H 8M5, Canada; Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa K1N 6N5, Canada; Canadian Institute for Advanced Research, Toronto, ON M5G 1M1, Canada.
  • MacKenzie AE; Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada.
  • Graber TE; Children's Hospital of Eastern Ontario Research Institute, Ottawa K1H 8L1, Canada.
  • Delatolla R; Department of Civil Engineering, University of Ottawa, Ottawa K1N 6N5, Canada. Electronic address: robert.delatolla@uOttawa.ca.
Water Res ; 188: 116560, 2021 Jan 01.
Article in English | MEDLINE | ID: covidwho-899651
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
In the absence of an effective vaccine to prevent COVID-19 it is important to be able to track community infections to inform public health interventions aimed at reducing the spread and therefore reduce pressures on health-care, improve health outcomes and reduce economic uncertainty. Wastewater surveillance has rapidly emerged as a potential tool to effectively monitor community infections through measuring trends of RNA signal in wastewater systems. In this study SARS-CoV-2 viral RNA N1 and N2 gene regions are quantified in solids collected from influent post grit solids (PGS) and primary clarified sludge (PCS) in two water resource recovery facilities (WRRF) serving Canada's national capital region, i.e., the City of Ottawa, ON (pop. ≈ 1.1M) and the City of Gatineau, QC (pop. ≈ 280K). PCS samples show signal inhibition using RT-ddPCR compared to RT-qPCR, with PGS samples showing similar quantifiable concentrations of RNA using both assays. RT-qPCR shows higher frequency of detection of N1 and N2 gene regions in PCS (92.7, 90.6%, n = 6) as compared to PGS samples (79.2, 82.3%, n = 5). Sampling of PCS may therefore be an effective approach for SARS-CoV-2 viral quantification, especially during periods of declining and low COVID-19 incidence in the community. The pepper mild mottle virus (PMMoV) is determined to have a less variable RNA signal in PCS over a three month period for two WRRFs, regardless of environmental conditions, compared to Bacteroides 16S rRNA or human 18S rRNA, making PMMoV a potentially useful biomarker for normalization of SARS-CoV-2 signal. PMMoV-normalized PCS RNA signal from WRRFs of two cities correlated with the regional public health epidemiological metrics, identifying PCS normalized to a fecal indicator (PMMoV) as a potentially effective tool for monitoring trends during decreasing and low-incidence of infection of SARS-Cov-2 in communities.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus / COVID-19 Type of study: Observational study Topics: Vaccines Limits: Humans Language: English Journal: Water Res Year: 2021 Document Type: Article Affiliation country: J.watres.2020.116560

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Betacoronavirus / COVID-19 Type of study: Observational study Topics: Vaccines Limits: Humans Language: English Journal: Water Res Year: 2021 Document Type: Article Affiliation country: J.watres.2020.116560