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Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods.
Bosnjak, Berislav; Stein, Saskia Catherina; Willenzon, Stefanie; Cordes, Anne Katrin; Puppe, Wolfram; Bernhardt, Günter; Ravens, Inga; Ritter, Christiane; Schultze-Florey, Christian R; Gödecke, Nina; Martens, Jörg; Kleine-Weber, Hannah; Hoffmann, Markus; Cossmann, Anne; Yilmaz, Mustafa; Pink, Isabelle; Hoeper, Marius M; Behrens, Georg M N; Pöhlmann, Stefan; Blasczyk, Rainer; Schulz, Thomas F; Förster, Reinhold.
  • Bosnjak B; Institute of Immunology, Hannover Medical School, Hannover, Germany. bosnjak.berislav@mh-hannover.de.
  • Stein SC; Institute of Virology, Hannover Medical School, Hannover, Germany.
  • Willenzon S; Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • Cordes AK; Institute of Virology, Hannover Medical School, Hannover, Germany.
  • Puppe W; Institute of Virology, Hannover Medical School, Hannover, Germany.
  • Bernhardt G; Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • Ravens I; Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • Ritter C; Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • Schultze-Florey CR; Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • Gödecke N; Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany.
  • Martens J; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Kleine-Weber H; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Hoffmann M; German Primate Center-Leibniz Institute for Primate Research, Göttingen, Germany.
  • Cossmann A; Faculty of Biology and Psychology, University Göttingen, Göttingen, Germany.
  • Yilmaz M; German Primate Center-Leibniz Institute for Primate Research, Göttingen, Germany.
  • Pink I; Faculty of Biology and Psychology, University Göttingen, Göttingen, Germany.
  • Hoeper MM; Department of Rheumatology and Immunology, Hannover Medical School, Hannover, Germany.
  • Behrens GMN; Public Health Department, Hannover, Germany.
  • Pöhlmann S; Department of Pneumology and German Centre of Lung Research (DZL), Hannover Medical School, Hannover, Germany.
  • Blasczyk R; Department of Pneumology and German Centre of Lung Research (DZL), Hannover Medical School, Hannover, Germany.
  • Schulz TF; Department of Rheumatology and Immunology, Hannover Medical School, Hannover, Germany.
  • Förster R; German Center for Infection Research (DZIF), partner site Hannover-Braunschweig, Hannover, Germany.
Cell Mol Immunol ; 18(4): 936-944, 2021 04.
Article in English | MEDLINE | ID: covidwho-899921
ABSTRACT
Neutralizing antibodies targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) block severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry into cells via surface-expressed angiotensin-converting enzyme 2 (ACE2). We used a surrogate virus neutralization test (sVNT) and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus (VSV) vector-based neutralization assay (pVNT) to assess the degree to which serum antibodies from coronavirus disease 2019 (COVID-19) convalescent patients interfere with the binding of SARS-CoV-2 S to ACE2. Both tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent patients. Importantly, sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA antibodies. Moreover, levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient age. Compared to pVNT, sVNT is less sophisticated and does not require any biosafety labs. Since this assay is also much faster and cheaper, sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / COVID-19 / Antibodies, Viral Type of study: Experimental Studies / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Cell Mol Immunol Journal subject: Allergy and Immunology Year: 2021 Document Type: Article Affiliation country: S41423-020-00573-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / COVID-19 / Antibodies, Viral Type of study: Experimental Studies / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Cell Mol Immunol Journal subject: Allergy and Immunology Year: 2021 Document Type: Article Affiliation country: S41423-020-00573-9