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Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse Model.
Abbas, Aymn T; El-Kafrawy, Sherif A; Sohrab, Sayed Sartaj; Tabll, Ashraf A; Hassan, Ahmed M; Iwata-Yoshikawa, Naoko; Nagata, Noriyo; Azhar, Esam I.
  • Abbas AT; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • El-Kafrawy SA; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Sohrab SS; Biotechnology Research Laboratories, Gastroenterology, Surgery Centre, Mansoura University, Mansoura 35511, Egypt.
  • Tabll AA; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Hassan AM; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Iwata-Yoshikawa N; Department of Clinical Pathology, National Liver Institute, Menoufiya University, Shebin El-Kom 32511, Egypt.
  • Nagata N; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Azhar EI; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Vaccines (Basel) ; 8(4)2020 Nov 01.
Article in English | MEDLINE | ID: covidwho-902683
Semantic information from SemMedBD (by NLM)
1. Anti-immunoglobulin antibody PREVENTS Pneumonia
Subject
Anti-immunoglobulin antibody
Predicate
PREVENTS
Object
Pneumonia
2. Borg Category-Ratio 10 Perceived Exertion Score 5 PROCESS_OF Homo sapiens
Subject
Borg Category-Ratio 10 Perceived Exertion Score 5
Predicate
PROCESS_OF
Object
Homo sapiens
3. Acute illness PROCESS_OF Homo sapiens
Subject
Acute illness
Predicate
PROCESS_OF
Object
Homo sapiens
4. Antibodies PART_OF yolk
Subject
Antibodies
Predicate
PART_OF
Object
yolk
5. M Protei PART_OF C3698360
Subject
M Protei
Predicate
PART_OF
Object
C3698360
6. yolk PART_OF Ovum
Subject
yolk
Predicate
PART_OF
Object
Ovum
7. Recombinant Proteins PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
Recombinant Proteins
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
8. Fluorescent Antibody Technique DIAGNOSES Middle East Respiratory Syndrome Coronavirus
Subject
Fluorescent Antibody Technique
Predicate
DIAGNOSES
Object
Middle East Respiratory Syndrome Coronavirus
9. Lung LOCATION_OF Anti-Antibodies
Subject
Lung
Predicate
LOCATION_OF
Object
Anti-Antibodies
10. Lung PART_OF Mus
Subject
Lung
Predicate
PART_OF
Object
Mus
11. Vero Cells LOCATION_OF Middle East Respiratory Syndrome Coronavirus
Subject
Vero Cells
Predicate
LOCATION_OF
Object
Middle East Respiratory Syndrome Coronavirus
12. Microneutralization Assay DIAGNOSES Weight decreased
Subject
Microneutralization Assay
Predicate
DIAGNOSES
Object
Weight decreased
13. Antigen ASSOCIATED_WITH C0021368
Subject
Antigen
Predicate
ASSOCIATED_WITH
Object
C0021368
14. Anti-immunoglobulin antibody PREVENTS Pneumonia
Subject
Anti-immunoglobulin antibody
Predicate
PREVENTS
Object
Pneumonia
15. Borg Category-Ratio 10 Perceived Exertion Score 5 PROCESS_OF Homo sapiens
Subject
Borg Category-Ratio 10 Perceived Exertion Score 5
Predicate
PROCESS_OF
Object
Homo sapiens
16. Acute illness PROCESS_OF Homo sapiens
Subject
Acute illness
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PROCESS_OF
Object
Homo sapiens
17. Antibodies PART_OF yolk
Subject
Antibodies
Predicate
PART_OF
Object
yolk
18. M Protein, multiple myeloma PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
M Protein, multiple myeloma
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
19. yolk PART_OF Ovum
Subject
yolk
Predicate
PART_OF
Object
Ovum
20. Recombinant Proteins PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
Recombinant Proteins
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
21. Fluorescent Antibody Technique DIAGNOSES Middle East Respiratory Syndrome Coronavirus
Subject
Fluorescent Antibody Technique
Predicate
DIAGNOSES
Object
Middle East Respiratory Syndrome Coronavirus
22. Lung LOCATION_OF Anti-Antibodies
Subject
Lung
Predicate
LOCATION_OF
Object
Anti-Antibodies
23. Lung PART_OF Mus
Subject
Lung
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PART_OF
Object
Mus
24. Vero Cells LOCATION_OF Middle East Respiratory Syndrome Coronavirus
Subject
Vero Cells
Predicate
LOCATION_OF
Object
Middle East Respiratory Syndrome Coronavirus
25. Microneutralization Assay DIAGNOSES Weight decreased
Subject
Microneutralization Assay
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DIAGNOSES
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Weight decreased
26. Antigens, Viral ASSOCIATED_WITH Inflammation
Subject
Antigens, Viral
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ASSOCIATED_WITH
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Inflammation
ABSTRACT
The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 and causes severe and often fatal acute respiratory illness in humans. No approved prophylactic and therapeutic interventions are currently available. In this study, we have developed egg yolk antibodies (immunoglobulin Y (IgY)) specific for MERS-CoV spike protein (S1) in order to evaluate their neutralizing efficiency against MERS-CoV infection. S1-specific immunoglobulins were produced by injecting chickens with purified recombinant S1 protein of MERS-CoV at a high titer (5.7 mg/mL egg yolk) at week 7 post immunization. Western blotting and immune-dot blot assays demonstrated that the IgY antibody specifically bound to the MERS-CoV S1 protein. Anti-S1 antibodies were also able to recognize MERS-COV inside cells, as demonstrated by an immunofluorescence assay. Plaque reduction and microneutralization assays showed the neutralization of MERS-COV in Vero cells by anti-S1 IgY antibodies and non-significantly reduced virus titers in the lungs of MERS-CoV-infected mice during early infection, with a nonsignificant decrease in weight loss. However, a statistically significant (p = 0.0196) quantitative reduction in viral antigen expression and marked reduction in inflammation were observed in lung tissue. Collectively, our data suggest that the anti-MERS-CoV S1 IgY could serve as a potential candidate for the passive treatment of MERS-CoV infection.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2020 Document Type: Article Affiliation country: Vaccines8040634

Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2020 Document Type: Article Affiliation country: Vaccines8040634