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Development and Optimization of In-house ELISA for Detection of Human IgG Antibody to SARS-CoV-2 Full Length Spike Protein.
Alandijany, Thamir A; El-Kafrawy, Sherif A; Tolah, Ahmed M; Sohrab, Sayed S; Faizo, Arwa A; Hassan, Ahmed M; Alsubhi, Tagreed L; Othman, Norah A; Azhar, Esam I.
  • Alandijany TA; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia.
  • El-Kafrawy SA; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Tolah AM; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia.
  • Sohrab SS; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Faizo AA; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia.
  • Hassan AM; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia.
  • Alsubhi TL; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
  • Othman NA; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 128442, Jeddah 21362, Saudi Arabia.
  • Azhar EI; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80324, Jeddah 21589, Saudi Arabia.
Pathogens ; 9(10)2020 Sep 28.
Article in English | MEDLINE | ID: covidwho-904941
Semantic information from SemMedBD (by NLM)
1. Enzyme Immunoassay METHOD_OF Detection
Subject
Enzyme Immunoassay
Predicate
METHOD_OF
Object
Detection
2. Circulatory Process PROCESS_OF Community
Subject
Circulatory Process
Predicate
PROCESS_OF
Object
Community
3. Serum PART_OF Homo sapiens
Subject
Serum
Predicate
PART_OF
Object
Homo sapiens
4. Serum LOCATION_OF Antibodie
Subject
Serum
Predicate
LOCATION_OF
Object
Antibodie
5. Enzyme Immunoassay METHOD_OF Detection
Subject
Enzyme Immunoassay
Predicate
METHOD_OF
Object
Detection
6. Circulatory Process PROCESS_OF Community
Subject
Circulatory Process
Predicate
PROCESS_OF
Object
Community
7. Serum PART_OF Homo sapiens
Subject
Serum
Predicate
PART_OF
Object
Homo sapiens
8. Serum LOCATION_OF Antibodies, Viral
Subject
Serum
Predicate
LOCATION_OF
Object
Antibodies, Viral
ABSTRACT
The ongoing coronavirus disease 19 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to human health. Despite this, many affected countries are now in the process of gradual lifting of COVID-19 restrictions that were initially implemented in response to the pandemic. The success of the so-called "exit strategy" requires continued surveillance of virus circulation in the community and evaluation of the prevalence of protective immunity among population. Serology tests are valuable tools for these purposes. Herein, SARS-CoV-2 full-length spike (S) recombinant protein was utilized to develop and optimize an indirect enzyme-linked immunoassay (ELISA) that enables a reliable detection of virus-specific IgG antibody in human sera. Importantly, the performance of this assay was evaluated utilizing micro-neutralization (MN) assay as a reference test. Our developed ELISA offers 100% sensitivity, 98.4% specificity, 98.8% agreement, and high overall accuracy. Moreover, the optical density (OD) values of positive samples significantly correlated with their MN titers. The assay specifically detects human IgG antibodies directed against SARS-CoV-2, but not those to Middle East respiratory syndrome coronavirus (MERS-CoV) or human coronavirus HKU1 (HCoV-HKU1). The availability of this in-house ELISA protocol would be valuable for various diagnostic and epidemiological applications.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Clinical Practice Guide / Risk factors Language: English Year: 2020 Document Type: Article Affiliation country: Pathogens9100803

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Clinical Practice Guide / Risk factors Language: English Year: 2020 Document Type: Article Affiliation country: Pathogens9100803