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Digital PCR is a sensitive new technique for SARS-CoV-2 detection in clinical applications.
Jiang, Yanfang; Wang, Haifeng; Hao, Sijia; Chen, Yukun; He, Jiaxue; Liu, Yong; Chen, Liguo; Yu, Yuanhua; Hua, Shucheng.
  • Jiang Y; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: yanfangjiang@hotmail.com.
  • Wang H; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: wanghaifeng1994@163.com.
  • Hao S; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: hsj.jdyy@outlook.com.
  • Chen Y; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: cyk0503@qq.com.
  • He J; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: jiaxue-he@163.com.
  • Liu Y; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: 530440980@qq.com.
  • Chen L; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: 602105535@qq.com.
  • Yu Y; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, China. Electronic address: yuyuanhua8888@126.com.
  • Hua S; Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address: huashucheng2020@163.com.
Clin Chim Acta ; 511: 346-351, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-907115
ABSTRACT
The global coronavirus disease 2019 (COVID-19) pandemic has posed great challenges in people's daily lives. Highly sensitive laboratory techniques played a critical role in clinical COVID-19 diagnosis and management. In this study the feasibility of using a new digital PCR-based detection assay for clinical COVID-19 diagnosis was investigated by comparing its performance with that of RT-PCR. Clinical patient samples and samples obtained from potentially contaminated environments were analyzed. The study included 10 patients with confirmed COVID-19 diagnoses, 32 validated samples of various types derived from different clinical timepoints and sites, and 148 environmentally derived samples. SARS-CoV-2 nucleic acids were more readily detected in respiratory tract samples (35.0%). In analyses of environmentally derived samples, the positivity rate of air samples was higher than that of surface samples, probably due to differences in virus concentrations. Digital PCR detected SARS-CoV-2 in several samples that had previously been deemed negative, including 3 patient-derived samples and 5 environmentally derived samples. In this study digital PCR exhibited higher sensitivity than conventional RT-PCR, suggesting that it may be a useful new method for clinical SARS-CoV-2 detection. Improvement of SARS-CoV-2 detection would substantially reduce the rates of false-negative COVID-19 test results, in particular those pertaining to asymptomatic carriers.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / Digital Technology / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Clin Chim Acta Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / Digital Technology / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Clin Chim Acta Year: 2020 Document Type: Article