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Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID-19.
Yan, Lin; Cai, Bei; Li, Yi; Wang, Min-Jin; An, Yun-Fei; Deng, Rong; Li, Dong-Dong; Wang, Li-Chun; Xu, Huan; Gao, Xue-Dan; Wang, Lan-Lan.
  • Yan L; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Cai B; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Li Y; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Wang MJ; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • An YF; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Deng R; Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
  • Li DD; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Wang LC; Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
  • Xu H; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Gao XD; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
  • Wang LL; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
J Cell Mol Med ; 24(24): 14270-14279, 2020 12.
Article in English | MEDLINE | ID: covidwho-907630
ABSTRACT
Recent studies have demonstrated a marked decrease in peripheral lymphocyte levels in patients with coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Few studies have focused on the changes of NK, T- and B-cell subsets, inflammatory cytokines and virus-specific antibodies in patients with moderate COVID-19. A total of 11 RT-PCR-confirmed convalescent patients with COVID-19 and 11 patients with non-SARS-CoV-2 pneumonia (control patients) were enrolled in this study. NK, CD8+ T, CD4+ T, Tfh-like and B-cell subsets were analysed using flow cytometry. Cytokines and SARS-CoV-2-specific antibodies were analysed using an electrochemiluminescence immunoassay. NK cell counts were significantly higher in patients with COVID-19 than in control patients (P = 0.017). Effector memory CD8+ T-cell counts significantly increased in patients with COVID-19 during a convalescent period of 1 week (P = 0.041). TIM-3+ Tfh-like cell and CD226+ Tfh-like cell counts significantly increased (P = 0.027) and decreased (P = 0.022), respectively, during the same period. Moreover, ICOS+ Tfh-like cell counts tended to decrease (P = 0.074). No abnormal increase in cytokine levels was observed. The high expression of NK cells is important in innate immune response against SARS-CoV-2. The increase in effector memory CD8+ T-cell counts, the up-regulation of inhibitory molecules and the down-regulation of active molecules on CD4+ T cells and Tfh-like cells in patients with COVID-19 would benefit the maintenance of balanced cellular and humoural immune responses, may prevent the development of severe cases and contribute to the recovery of patients with COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Killer Cells, Natural / Cytokines / CD8-Positive T-Lymphocytes / T Follicular Helper Cells / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: J Cell Mol Med Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: Jcmm.16044

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Killer Cells, Natural / Cytokines / CD8-Positive T-Lymphocytes / T Follicular Helper Cells / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: J Cell Mol Med Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: Jcmm.16044