Label-free optical antibody testing kit based on a self-assembled whispering-gallery-mode microsphere.
J Biophotonics
; 14(3): e202000338, 2021 03.
Article
in English
| MEDLINE | ID: covidwho-908749
ABSTRACT
The appearance of antibodies in blood is a critical signal to suggest the infection. A rapid and accurate detection method for the antibody is significant to the disease diagnosis, especially for the epidemic. To this end, a highly sensitive whispering-gallery-mode (WGM) optical testing kit is designed and fabricated for detecting the specific immunoglobulin antibodies. The key component of the kit is a silica self-assembled microsphere decorated with the nucleocapsid proteins (N-proteins) of the SARS-CoV-2 virus. After the N-protein antibody immunoglobulin G (N-IgG) and immunoglobulin M (N-IgM) solutions being injected into the kit, the WGM red-shifts due to the antigen-antibody reaction. The wavelength displacement rates are proportional to the concentrations of these two antibodies from 1 to 100 µg/mL. A good specificity of the kit is demonstrated by the nonspecific human immunoglobulin G (H-IgG) and immunoglobulin M (H-IgM).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Silicon Dioxide
/
COVID-19 Serological Testing
/
COVID-19
/
Microspheres
/
Antibodies, Viral
Type of study:
Diagnostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
J Biophotonics
Journal subject:
Biophysics
Year:
2021
Document Type:
Article
Affiliation country:
Jbio.202000338
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