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Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein.
Tandon, Ritesh; Mitra, Dipanwita; Sharma, Poonam; McCandless, Martin G; Stray, Stephen J; Bates, John T; Marshall, Gailen D.
  • Tandon R; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA. rtandon@umc.edu.
  • Mitra D; Department of Medicine, University of Mississippi Medical Center, Jackson, MS, 39216, USA. rtandon@umc.edu.
  • Sharma P; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
  • McCandless MG; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
  • Stray SJ; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
  • Bates JT; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
  • Marshall GD; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, 39216, USA.
Sci Rep ; 10(1): 19076, 2020 11 05.
Article in English | MEDLINE | ID: covidwho-912909
ABSTRACT
Pseuodotyped particles have significant importance and use in virology as tools for studying the biology of highly pathogenic viruses in a lower biosafety environment. The biological, chemical, and serological studies of the recently emerged SARS-CoV-2 will be greatly aided by the development and optimization of a suitable pseudotyping system. Here, we pseudotyped the SARS-CoV-2 Spike glycoprotein (SPG) on a traditional retroviral (MMLV) as well as a third generation lentiviral (pLV) vector and tested the transduction efficiency in several mammalian cell lines expressing SARS-CoV-2 receptor hACE2. While MMLV pseudotyped the vesicular stomatitis virus G glycoprotein (VSV-G) efficiently, it could not pseudotype the full-length SPG. In contrast, pLV pseudotyped both glycoproteins efficiently; however, much higher titers of pLV-G particles were produced. Among all the tested mammalian cells, 293Ts expressing hACE2 were most efficiently transduced using the pLV-S system. The pLV-S particles were efficiently neutralized by diluted serum (>640) from recently recovered COVID-19 patients who showed high SARS-CoV-2 specific IgM and IgG levels. In summary, pLV-S pseudotyped virus provides a valid screening tool for the presence of anti SARS-CoV-2 specific neutralizing antibodies in convalescent patient serum.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Serologic Tests / Lentivirus / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies Limits: Animals / Humans Language: English Journal: Sci Rep Year: 2020 Document Type: Article Affiliation country: S41598-020-76135-w

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Serologic Tests / Lentivirus / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies Limits: Animals / Humans Language: English Journal: Sci Rep Year: 2020 Document Type: Article Affiliation country: S41598-020-76135-w