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Preexisting and de novo humoral immunity to SARS-CoV-2 in humans.
Ng, Kevin W; Faulkner, Nikhil; Cornish, Georgina H; Rosa, Annachiara; Harvey, Ruth; Hussain, Saira; Ulferts, Rachel; Earl, Christopher; Wrobel, Antoni G; Benton, Donald J; Roustan, Chloe; Bolland, William; Thompson, Rachael; Agua-Doce, Ana; Hobson, Philip; Heaney, Judith; Rickman, Hannah; Paraskevopoulou, Stavroula; Houlihan, Catherine F; Thomson, Kirsty; Sanchez, Emilie; Shin, Gee Yen; Spyer, Moira J; Joshi, Dhira; O'Reilly, Nicola; Walker, Philip A; Kjaer, Svend; Riddell, Andrew; Moore, Catherine; Jebson, Bethany R; Wilkinson, Meredyth; Marshall, Lucy R; Rosser, Elizabeth C; Radziszewska, Anna; Peckham, Hannah; Ciurtin, Coziana; Wedderburn, Lucy R; Beale, Rupert; Swanton, Charles; Gandhi, Sonia; Stockinger, Brigitta; McCauley, John; Gamblin, Steve J; McCoy, Laura E; Cherepanov, Peter; Nastouli, Eleni; Kassiotis, George.
  • Ng KW; Retroviral Immunology, The Francis Crick Institute, London NW1 1AT, UK.
  • Faulkner N; Retroviral Immunology, The Francis Crick Institute, London NW1 1AT, UK.
  • Cornish GH; Retroviral Immunology, The Francis Crick Institute, London NW1 1AT, UK.
  • Rosa A; Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Harvey R; Worldwide Influenza Centre, The Francis Crick Institute, London NW1 1AT, UK.
  • Hussain S; Worldwide Influenza Centre, The Francis Crick Institute, London NW1 1AT, UK.
  • Ulferts R; Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Earl C; Signalling and Structural Biology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Wrobel AG; Structural Biology of Disease Processes Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Benton DJ; Structural Biology of Disease Processes Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Roustan C; Structural Biology STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Bolland W; Retroviral Immunology, The Francis Crick Institute, London NW1 1AT, UK.
  • Thompson R; Retroviral Immunology, The Francis Crick Institute, London NW1 1AT, UK.
  • Agua-Doce A; Flow Cytometry STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Hobson P; Flow Cytometry STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Heaney J; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Rickman H; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Paraskevopoulou S; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Houlihan CF; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Thomson K; Division of Infection and Immunity, University College London (UCL), London WC1E 6BT, UK.
  • Sanchez E; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Shin GY; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Spyer MJ; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • Joshi D; University College London Hospitals (UCLH) NHS Trust, London NW1 2BU, UK.
  • O'Reilly N; Department of Population, Policy and Practice, Great Ormond Street Institute for Child Health (ICH), UCL, London WC1N 1EH, UK.
  • Walker PA; Peptide Chemistry, The Francis Crick Institute, London NW1 1AT, UK.
  • Kjaer S; Peptide Chemistry, The Francis Crick Institute, London NW1 1AT, UK.
  • Riddell A; Structural Biology STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Moore C; Structural Biology STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Jebson BR; Flow Cytometry STP, The Francis Crick Institute, London NW1 1AT, UK.
  • Wilkinson M; Public Health Wales, University Hospital of Wales, Cardiff CF14 4XW, UK.
  • Marshall LR; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Rosser EC; UCL Great Ormond Street Institute for Child Health (ICH), UCL, London WC1N 1EH, UK.
  • Radziszewska A; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Peckham H; UCL Great Ormond Street Institute for Child Health (ICH), UCL, London WC1N 1EH, UK.
  • Ciurtin C; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Wedderburn LR; UCL Great Ormond Street Institute for Child Health (ICH), UCL, London WC1N 1EH, UK.
  • Beale R; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Swanton C; Centre for Rheumatology Research, Division of Medicine, UCL, London, WC1E 6BT, UK.
  • Gandhi S; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Stockinger B; Centre for Rheumatology Research, Division of Medicine, UCL, London, WC1E 6BT, UK.
  • McCauley J; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Gamblin SJ; Centre for Rheumatology Research, Division of Medicine, UCL, London, WC1E 6BT, UK.
  • McCoy LE; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Cherepanov P; Centre for Rheumatology Research, Division of Medicine, UCL, London, WC1E 6BT, UK.
  • Nastouli E; Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH, Great Ormond Street Hospital (GOSH), London WC1N 3JH, UK.
  • Kassiotis G; UCL Great Ormond Street Institute for Child Health (ICH), UCL, London WC1N 1EH, UK.
Science ; 370(6522): 1339-1343, 2020 12 11.
Article in English | MEDLINE | ID: covidwho-913669
Semantic information from SemMedBD (by NLM)
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ABSTRACT
Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunity, Humoral / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abe1107

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunity, Humoral / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abe1107