Your browser doesn't support javascript.
Interleukin-1ß Modulation of the Mechanobiology of Primary Human Pulmonary Fibroblasts: Potential Implications in Lung Repair.
Gabasa, Marta; Arshakyan, Marselina; Llorente, Alejandro; Chuliá-Peris, Lourdes; Pavelescu, Irina; Xaubet, Antoni; Pereda, Javier; Alcaraz, Jordi.
  • Gabasa M; Unit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain.
  • Arshakyan M; Unit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain.
  • Llorente A; Unit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain.
  • Chuliá-Peris L; Departament of Physiology, Faculty of Pharmacy, Universitat de València, 46100 València, Spain.
  • Pavelescu I; Unit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain.
  • Xaubet A; Pneumology Service, Hospital Clínic, 08036 Barcelona, Spain.
  • Pereda J; Departament of Physiology, Faculty of Pharmacy, Universitat de València, 46100 València, Spain.
  • Alcaraz J; Unit of Biophysics and Bioengineering, Department of Biomedicine, School of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain.
Int J Mol Sci ; 21(22)2020 Nov 10.
Article in English | MEDLINE | ID: covidwho-917002
Semantic information from SemMedBD (by NLM)
1. Fibroblasts PART_OF Lung
Subject
Fibroblasts
Predicate
PART_OF
Object
Lung
2. interleukin- AFFECTS C0376706
Subject
interleukin-
Predicate
AFFECTS
Object
C0376706
3. Down-Regulation PROCESS_OF Fibroblasts
Subject
Down-Regulation
Predicate
PROCESS_OF
Object
Fibroblasts
4. interleukin- PREVENTS C0427008
Subject
interleukin-
Predicate
PREVENTS
Object
C0427008
5. interleukin- PART_OF C0013018
Subject
interleukin-
Predicate
PART_OF
Object
C0013018
6. interleukin- DISRUPTS C0026820
Subject
interleukin-
Predicate
DISRUPTS
Object
C0026820
7. Fibroblasts PART_OF Lung
Subject
Fibroblasts
Predicate
PART_OF
Object
Lung
8. interleukin-1, beta AFFECTS Mechanics
Subject
interleukin-1, beta
Predicate
AFFECTS
Object
Mechanics
9. Down-Regulation PROCESS_OF Fibroblasts
Subject
Down-Regulation
Predicate
PROCESS_OF
Object
Fibroblasts
10. interleukin-1, beta PREVENTS Stiffness
Subject
interleukin-1, beta
Predicate
PREVENTS
Object
Stiffness
11. interleukin-1, beta PART_OF Donor person
Subject
interleukin-1, beta
Predicate
PART_OF
Object
Donor person
12. interleukin-1, beta DISRUPTS Muscle Contraction
Subject
interleukin-1, beta
Predicate
DISRUPTS
Object
Muscle Contraction
ABSTRACT
Pro-inflammatory cytokines like interleukin-1ß (IL-1ß) are upregulated during early responses to tissue damage and are expected to transiently compromise the mechanical microenvironment. Fibroblasts are key regulators of tissue mechanics in the lungs and other organs. However, the effects of IL-1ß on fibroblast mechanics and functions remain unclear. Here we treated human pulmonary fibroblasts from control donors with IL-1ß and used Atomic Force Microscopy to unveil that IL-1ß significantly reduces the stiffness of fibroblasts concomitantly with a downregulation of filamentous actin (F-actin) and alpha-smooth muscle (α-SMA). Likewise, COL1A1 mRNA was reduced, whereas that of collagenases MMP1 and MMP2 were upregulated, favoring a reduction of type-I collagen. These mechanobiology changes were functionally associated with reduced proliferation and enhanced migration upon IL-1ß stimulation, which could facilitate lung repair by drawing fibroblasts to sites of tissue damage. Our observations reveal that IL-1ß may reduce local tissue rigidity by acting both intracellularly and extracellularly through the downregulation of fibroblast contractility and type I collagen deposition, respectively. These IL-1ß-dependent mechanical effects may enhance lung repair further by locally increasing pulmonary tissue compliance to preserve normal lung distension and function. Moreover, our results support that IL-1ß provides innate anti-fibrotic protection that may be relevant during the early stages of lung repair.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Interleukin-1beta / Lung Language: English Year: 2020 Document Type: Article Affiliation country: Ijms21228417

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Interleukin-1beta / Lung Language: English Year: 2020 Document Type: Article Affiliation country: Ijms21228417