Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation.
Bioprocess Biosyst Eng
; 44(4): 653-660, 2021 Apr.
Article
in English
| MEDLINE | ID: covidwho-917119
ABSTRACT
Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be promising anti-COVID-19 agents. However, conventional cultivation of MA does not meet the demand for its stilbenes, and root quality usually varies between crops. This study established the in vitro non-GMO root culture of MA and optimized the root density, precursor feeding, and elicitors for stilbene productivity. A root culture with optimal inoculum density (3 g/flask of 30 mL medium) accumulated mulberroside A, oxyresveratrol, and resveratrol at 18.7 ± 1.00 mg/g, 136 ± 5.05 µg/g, and 41.6 ± 5.84 µg/g dry weight (DW), respectively. The feeding of L-tyrosine shortened the time required to reach the stilbene productive stage. Root cultures co-treated with 200 µM methyl jasmonate and 2 mg/mL yeast extract accumulated the highest contents of mulberroside A (30.3 ± 2.68 mg/g DW), oxyresveratrol (68.6 ± 3.53 µg/g DW), and resveratrol (10.2 ± 0.53 µg/g DW). In summary, root culture is a promising and sustainable source of stilbenes for the development of health products and agents for further investigation as potential anti-COVID-19 agents.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Stilbenes
/
Plant Roots
/
Morus
/
Plant Cells
Limits:
Humans
Language:
English
Journal:
Bioprocess Biosyst Eng
Journal subject:
Biotechnology
/
Biomedical Engineering
Year:
2021
Document Type:
Article
Affiliation country:
S00449-020-02474-7
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