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A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals.
Zhao, Zhangting; Ma, Xingyuan; Zhang, Ruihuan; Hu, Fabiao; Zhang, Tong; Liu, Yuping; Han, Myong Hun; You, Fang; Yang, Yi; Zheng, Wenyun.
  • Zhao Z; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
  • Ma X; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
  • Zhang R; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
  • Hu F; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
  • Zhang T; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
  • Liu Y; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
  • Han MH; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China; Department of Genetics, Faculty of Life Science, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.
  • You F; Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore.
  • Yang Y; SinGENE Biotech Pte Ltd, Singapore Science Park, Singapore 118258, Singapore. Electronic address: yangyi@singenebio.com.
  • Zheng W; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, PR China. Electronic address: zwy@ecust.edu.edu.cn.
Nanomedicine ; 35: 102338, 2021 07.
Article in English | MEDLINE | ID: covidwho-921611
ABSTRACT
DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61 ±â€¯15.63 nm) and high encapsulation efficiency (87.60 ±â€¯8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58- and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, DNA / DNA Replication / Nanoparticles / Immunity, Humoral / Immunity, Cellular / Epitopes Type of study: Experimental Studies / Prognostic study Topics: Vaccines Limits: Animals Language: English Journal: Nanomedicine Journal subject: Biotechnology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, DNA / DNA Replication / Nanoparticles / Immunity, Humoral / Immunity, Cellular / Epitopes Type of study: Experimental Studies / Prognostic study Topics: Vaccines Limits: Animals Language: English Journal: Nanomedicine Journal subject: Biotechnology Year: 2021 Document Type: Article