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Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads.
Wang, Chongwen; Yang, Xingsheng; Gu, Bing; Liu, Haifeng; Zhou, Zihui; Shi, Luoluo; Cheng, Xiaodan; Wang, Shengqi.
  • Wang C; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
  • Yang X; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Gu B; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
  • Liu H; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Zhou Z; Medical Technology Institute of Xuzhou Medical University, Xuzhou 221004, P. R. China.
  • Shi L; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, P. R. China.
  • Cheng X; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
  • Wang S; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
Anal Chem ; 92(23): 15542-15549, 2020 12 01.
Article in English | MEDLINE | ID: covidwho-933643
ABSTRACT
A rapid and accurate method for detection of virus (SARS-CoV-2)-specific antibodies is important to contain the 2019 coronavirus disease (COVID-19) outbreak, which is still urgently needed. Here, we develop a colorimetric-fluorescent dual-mode lateral flow immunoassay (LFIA) biosensor for rapid, sensitive, and simultaneous detection of SARS-CoV-2-specific IgM and IgG in human serum using spike (S) protein-conjugated SiO2@Au@QD nanobeads (NBs) as labels. The assay only needs 1 µL of the serum sample, can be completed within 15 min, and is 100 times more sensitive than the colloidal gold-based LFIA. Two detection modes of our biosensor are available the colorimetric mode for rapid screening of the patients with suspected SARS-CoV-2 infection without any special instrument and the fluorescent mode for sensitive and quantitative analyses to determine the concentrations of specific IgM/IgG in human serum and detect the infection early and precisely. We validated the proposed method using 16 positive serum samples from patients with COVID-19 and 41 negative samples from patients with other viral respiratory infections. The results demonstrated that combined detection of virus-specific IgM and IgG via SiO2@Au@QD LFIA can identify 100% of patients with SARS-CoV-2 infection with 100% specificity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Immunoglobulin M / Immunoassay / Quantum Dots / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Anal Chem Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Immunoglobulin M / Immunoassay / Quantum Dots / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Anal Chem Year: 2020 Document Type: Article