Impact of Pool Testing in Detection of Asymptomatic Patients with COVID-19.
Lab Med
; 52(1): e15-e16, 2021 Jan 04.
Article
in English
| MEDLINE | ID: covidwho-933866
ABSTRACT
OBJECTIVE:
During the current pandemic, COVID-19 has been detected in patients using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) that confirms the presence of SARS-CoV-2 RNA. The demand for increased testing, particularly for asymptomatic individuals required alternative approaches to single-patient RT-PCR testing, such as pooling.METHODS:
This study explored the impact of dilution on the detectability of SARS-CoV-2 in asymptomatic patients using RT-PCR and demonstrated that pooling can be effective in low prevalence populations.RESULTS:
The RT-PCR results for the 31, 51, and 71 aliquot samples showed little differences in CT values, confirming detection capability at these dilutions.CONCLUSION:
Based on the results of the present study, a pooled approach with up to 51 sample aliquots and using the current RT-PCR methodology likely will detect SARS CoV2 RNA among asymptomatic patients.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA, Viral
/
Reverse Transcriptase Polymerase Chain Reaction
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Observational study
Limits:
Humans
Language:
English
Journal:
Lab Med
Year:
2021
Document Type:
Article
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