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Polymer microarrays rapidly identify competitive adsorbents of virus-like particles.
Blok, Andrew J; Gurnani, Pratik; Xenopoulos, Alex; Burroughs, Laurence; Duncan, Joshua; Urbanowicz, Richard A; Tsoleridis, Theocharis; Müller-Kräuter, Helena; Strecker, Thomas; Ball, Jonathan K; Alexander, Cameron; Alexander, Morgan R.
  • Blok AJ; Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Gurnani P; Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Xenopoulos A; EMD Millipore, 80 Ashby Road, Bedford, Massachusetts 01730.
  • Burroughs L; Division of Advanced Materials and Healthcare Technologies, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Duncan J; Wolfson Centre for Global Virus Research, Faculty of Medicine and Health Science, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Urbanowicz RA; Wolfson Centre for Global Virus Research, Faculty of Medicine and Health Science, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Tsoleridis T; Wolfson Centre for Global Virus Research, Faculty of Medicine and Health Science, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Müller-Kräuter H; Institute of Virology, Philipps University of Marburg, Marburg 35043, Germany.
  • Strecker T; Institute of Virology, Philipps University of Marburg, Marburg 35043, Germany.
  • Ball JK; Wolfson Centre for Global Virus Research, Faculty of Medicine and Health Science, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Alexander C; Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
  • Alexander MR; Division of Advanced Materials and Healthcare Technologies, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
Biointerphases ; 15(6): 061005, 2020 11 17.
Article in English | MEDLINE | ID: covidwho-934052
ABSTRACT
The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable the rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechanistic knowledge of specific material-virion interactions before they can be employed, for example, to aid in the purification of vaccine components or in the design of a more effective PPE. Here, we show that an adaption of a polymer microarray method for screening bacterial-surface interactions allows for the screening of polymers for desirable material-virion interactions. Nonpathogenic virus-like particles including fluorophores are exposed to the arrays in an aqueous buffer as a simple model of virions carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella virus-like particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for the discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus, which we propose would be part of a material scale up step carried out in high containment facilities, alongside the use of more complex media to represent biological fluids.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Polymers / Virion / Microarray Analysis Type of study: Diagnostic study Topics: Vaccines Language: English Journal: Biointerphases Journal subject: Biotechnology / Biomedical Engineering Year: 2020 Document Type: Article Affiliation country: 6.0000586

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Polymers / Virion / Microarray Analysis Type of study: Diagnostic study Topics: Vaccines Language: English Journal: Biointerphases Journal subject: Biotechnology / Biomedical Engineering Year: 2020 Document Type: Article Affiliation country: 6.0000586