Your browser doesn't support javascript.
SARS-CoV-2 Antibody Responses Are Correlated to Disease Severity in COVID-19 Convalescent Individuals.
Hansen, Cecilie Bo; Jarlhelt, Ida; Pérez-Alós, Laura; Hummelshøj Landsy, Lone; Loftager, Mette; Rosbjerg, Anne; Helgstrand, Charlotte; Bjelke, Jais Rose; Egebjerg, Thomas; Jardine, Joseph G; Sværke Jørgensen, Charlotte; Iversen, Kasper; Bayarri-Olmos, Rafael; Garred, Peter; Skjoedt, Mikkel-Ole.
  • Hansen CB; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Jarlhelt I; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Pérez-Alós L; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Hummelshøj Landsy L; Department of Non-Clinical and Clinical Assay Sciences, Global Discovery and Development Sciences, Novo Nordisk A/S, 2760 Måløv, Denmark.
  • Loftager M; Department of Non-Clinical and Clinical Assay Sciences, Global Discovery and Development Sciences, Novo Nordisk A/S, 2760 Måløv, Denmark.
  • Rosbjerg A; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Helgstrand C; Recombinant Technologies, Novo Nordisk A/S, 2760 Måløv, Denmark.
  • Bjelke JR; Recombinant Technologies, Novo Nordisk A/S, 2760 Måløv, Denmark.
  • Egebjerg T; Recombinant Technologies, Novo Nordisk A/S, 2760 Måløv, Denmark.
  • Jardine JG; IAVI Neutralizing Antibody Center, Scripps Research, La Jolla, CA 92037.
  • Sværke Jørgensen C; Department of Microbiological Diagnostics and Virology, Statens Serum Institut, 2300 Copenhagen, Denmark.
  • Iversen K; Department of Cardiology, Herlev University Hospital, 2730 Herlev, Denmark; and.
  • Bayarri-Olmos R; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Garred P; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
  • Skjoedt MO; Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; moskjoedt@sund.ku.dk.
J Immunol ; 206(1): 109-117, 2021 01 01.
Article in English | MEDLINE | ID: covidwho-934538
Semantic information from SemMedBD (by NLM)
1. Antibody Formation PROCESS_OF Persons
Subject
Antibody Formation
Predicate
PROCESS_OF
Object
Persons
2. DDX41 wt Allele|DDX41 PART_OF 2019 novel coronavirus
Subject
DDX41 wt Allele|DDX41
Predicate
PART_OF
Object
2019 novel coronavirus
3. Screening procedure METHOD_OF Diagnosis
Subject
Screening procedure
Predicate
METHOD_OF
Object
Diagnosis
4. Assay METHOD_OF Diagnosis
Subject
Assay
Predicate
METHOD_OF
Object
Diagnosis
5. Performance PROCESS_OF Cohort
Subject
Performance
Predicate
PROCESS_OF
Object
Cohort
6. Antibody Formation PROCESS_OF Persons
Subject
Antibody Formation
Predicate
PROCESS_OF
Object
Persons
7. DDX41 wt Allele|DDX41 PART_OF 2019 novel coronavirus
Subject
DDX41 wt Allele|DDX41
Predicate
PART_OF
Object
2019 novel coronavirus
8. Screening procedure METHOD_OF Diagnosis
Subject
Screening procedure
Predicate
METHOD_OF
Object
Diagnosis
9. Assay METHOD_OF Diagnosis
Subject
Assay
Predicate
METHOD_OF
Object
Diagnosis
10. Performance PROCESS_OF Cohort
Subject
Performance
Predicate
PROCESS_OF
Object
Cohort
ABSTRACT
Globally, the COVID-19 pandemic has had extreme consequences for the healthcare system and has led to calls for diagnostic tools to monitor and understand the transmission, pathogenesis, and epidemiology, as well as to evaluate future vaccination strategies. In this study, we have developed novel, to our knowledge, flexible ELISA-based assays for specific detection of human SARS-CoV-2 Abs against the receptor-binding domain, including an Ag sandwich ELISA relevant for large population screening and three isotype-specific assays for in-depth diagnostics. Their performance was evaluated in a cohort of 350 convalescent participants with previous COVID-19 infection, ranging from asymptomatic to critical cases. We mapped the Ab responses to different areas on protein N and S and showed that the IgM, A, and G Ab responses against receptor-binding domain are significantly correlated to the disease severity. These assays and the data generated from them are highly relevant for diagnostics and prognostics and contribute to the understanding of long-term COVID-19 immunity.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Convalescence / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Prognostic study Topics: Long Covid / Vaccines Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Immunol Year: 2021 Document Type: Article Affiliation country: Jimmunol.2000898

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Convalescence / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Prognostic study Topics: Long Covid / Vaccines Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Immunol Year: 2021 Document Type: Article Affiliation country: Jimmunol.2000898