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Nanozyme chemiluminescence paper test for rapid and sensitive detection of SARS-CoV-2 antigen.
Liu, Dan; Ju, Chenhui; Han, Chao; Shi, Rui; Chen, Xuehui; Duan, Demin; Yan, Jinghua; Yan, Xiyun.
  • Liu D; CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academic of Science, Beijing, 100101, China.
  • Ju C; CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academic of Science, Beijing, 100101, China.
  • Han C; CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Shi R; CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Chen X; CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academic of Science, Beijing, 100101, China.
  • Duan D; CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academic of Science, Beijing, 100101, China.
  • Yan J; CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Yan X; CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academic of Science, Beijing, 100101, China. Electronic address: yanxy@ibp.ac.cn.
Biosens Bioelectron ; 173: 112817, 2020 Nov 13.
Article in English | MEDLINE | ID: covidwho-935459
Semantic information from SemMedBD (by NLM)
1. Antigens PART_OF 2019 novel coronavirus
Subject
Antigens
Predicate
PART_OF
Object
2019 novel coronavirus
2. Early Diagnosis DIAGNOSES 2019 novel coronavirus
Subject
Early Diagnosis
Predicate
DIAGNOSES
Object
2019 novel coronavirus
3. Serologic tests USES Antibodies
Subject
Serologic tests
Predicate
USES
Object
Antibodies
4. Enzyme Immunoassay DIAGNOSES Hypersensitivity
Subject
Enzyme Immunoassay
Predicate
DIAGNOSES
Object
Hypersensitivity
5. Antigens PART_OF 2019 novel coronavirus
Subject
Antigens
Predicate
PART_OF
Object
2019 novel coronavirus
6. Early Diagnosis DIAGNOSES 2019 novel coronavirus
Subject
Early Diagnosis
Predicate
DIAGNOSES
Object
2019 novel coronavirus
7. Serologic tests USES Antibodies
Subject
Serologic tests
Predicate
USES
Object
Antibodies
8. Enzyme Immunoassay DIAGNOSES Hypersensitivity
Subject
Enzyme Immunoassay
Predicate
DIAGNOSES
Object
Hypersensitivity
ABSTRACT
COVID-19 has evolved into a global pandemic. Early and rapid detection is crucial to control of the SARS-CoV-2 transmission. While representing the gold standard for early diagnosis, nucleic acid tests for SARS-CoV-2 are often complicated and time-consuming. Serological rapid antibody tests are characterized by high rates of false-negative diagnoses, especially during early infection. Here, we developed a novel nanozyme-based chemiluminescence paper assay for rapid and sensitive detection of SARS-CoV-2 spike antigen, which integrates nanozyme and enzymatic chemiluminescence immunoassay with the lateral flow strip. The core of our paper test is a robust Co-Fe@hemin-peroxidase nanozyme that catalyzes chemiluminescence comparable with natural peroxidase HRP and thus amplifies immune reaction signal. The detection limit for recombinant spike antigen of SARS-CoV-2 was 0.1 ng/mL, with a linear range of 0.2-100 ng/mL. Moreover, the sensitivity of test for pseudovirus could reach 360 TCID50/mL, which was comparable with ELISA method. The strip recognized SARS-CoV-2 antigen specifically, and there was no cross reaction with other coronaviruses or influenza A subtypes. This testing can be completed within 16 min, much shorter compared to the usual 1-2 h required for currently used nucleic acid tests. Furthermore, signal detection is feasible using the camera of a standard smartphone. Ingredients for nanozyme synthesis are simple and readily available, considerably lowering the overall cost. In conclusion, our paper test provides a high-sensitive point-of-care testing (POCT) approach for SARS-CoV-2 antigen detection, which should greatly facilitate early screening of SARS-CoV-2 infections, and considerably lower the financial burden on national healthcare resources.
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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Type of study: Diagnostic study / Screening study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Type of study: Diagnostic study / Screening study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2020
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