Increased sensitivity using real-time dPCR for detection of SARS-CoV-2.
Biotechniques
; 70(1): 7-20, 2021 01.
Article
in English
| MEDLINE | ID: covidwho-940124
ABSTRACT
A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Real-Time Polymerase Chain Reaction
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Biotechniques
Year:
2021
Document Type:
Article
Affiliation country:
Btn-2020-0133
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