Your browser doesn't support javascript.
Nasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.
Bricmont, Noemie; Benchimol, Lionel; Poirrier, Anne-Lise; Grignet, Christine; Seaton, Claire; Chilvers, Mark A; Seghaye, Marie-Christine; Louis, Renaud; Lefebvre, Philippe; Kempeneers, Celine.
  • Bricmont N; Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège; noemie.bricmont@uliege.be.
  • Benchimol L; Department of ENT, University Hospital of Liège.
  • Poirrier AL; Department of ENT, University Hospital of Liège.
  • Grignet C; Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège.
  • Seaton C; Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital.
  • Chilvers MA; Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital.
  • Seghaye MC; Division of Cardiology, Department of Pediatrics, University Hospital of Liège.
  • Louis R; Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège; Department of Pneumology, University Hospital of Liège.
  • Lefebvre P; Department of ENT, University Hospital of Liège.
  • Kempeneers C; Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège; Division of Respirology, Department of Pediatrics, University Hospital of Liège.
J Vis Exp ; (165)2020 11 07.
Article in English | MEDLINE | ID: covidwho-940257
ABSTRACT
Primary Ciliary Dyskinesia (PCD) is a genetic motile ciliopathy, leading to significant otosinopulmonary disease. PCD diagnosis is often missed or delayed due to challenges with different diagnostic modalities. Ciliary videomicroscopy, using Digital High-Speed Videomicroscopy (DHSV), one of the diagnostic tools for PCD, is considered the optimal method to perform ciliary functional analysis (CFA), comprising of ciliary beat frequency (CBF) and beat pattern (CBP) analysis. However, DHSV lacks standardized, published operating procedure for processing and analyzing samples. It also uses living respiratory epithelium, a significant infection control issue during the COVID-19 pandemic. To continue providing a diagnostic service during this health crisis, the ciliary videomicroscopy protocol has been adapted to include adequate infection control measures. Here, we describe a revised protocol for sampling and laboratory processing of ciliated respiratory samples, highlighting adaptations made to comply with COVID-19 infection control measures. Representative results of CFA from nasal brushing samples obtained from 16 healthy subjects, processed and analyzed according to this protocol, are described. We also illustrate the importance of obtaining and processing optimal quality epithelial ciliated strips, as samples not meeting quality selection criteria do now allow for CFA, potentially decreasing the diagnostic reliability and the efficiency of this technique.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Ciliary Motility Disorders / Infection Control / Coronavirus Infections / Pandemics / Betacoronavirus / Nasal Mucosa Type of study: Diagnostic study / Observational study Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Year: 2020 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Ciliary Motility Disorders / Infection Control / Coronavirus Infections / Pandemics / Betacoronavirus / Nasal Mucosa Type of study: Diagnostic study / Observational study Limits: Adult / Female / Humans / Male / Middle aged / Young adult Language: English Year: 2020 Document Type: Article