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Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platform.
Chiuppesi, Flavia; Salazar, Marcela d'Alincourt; Contreras, Heidi; Nguyen, Vu H; Martinez, Joy; Park, Yoonsuh; Nguyen, Jenny; Kha, Mindy; Iniguez, Angelina; Zhou, Qiao; Kaltcheva, Teodora; Levytskyy, Roman; Ebelt, Nancy D; Kang, Tae Hyuk; Wu, Xiwei; Rogers, Thomas F; Manuel, Edwin R; Shostak, Yuriy; Diamond, Don J; Wussow, Felix.
  • Chiuppesi F; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Salazar MD; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Contreras H; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Nguyen VH; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Martinez J; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Park Y; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Nguyen J; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Kha M; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Iniguez A; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Zhou Q; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Kaltcheva T; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Levytskyy R; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Ebelt ND; Department of Immuno-Oncology, Beckman Research Institute of the City of Hope, Duarte, CA, 91010, USA.
  • Kang TH; Integrative Genomics Core, Beckman Research Institute of the City of Hope, Duarte, CA, 91010, USA.
  • Wu X; Integrative Genomics Core, Beckman Research Institute of the City of Hope, Duarte, CA, 91010, USA.
  • Rogers TF; Division of Infectious Diseases and Global Public Health, University of California San Diego, School of Medicine, 9500 Gilman Dr, La Jolla, CA, 92093, USA.
  • Manuel ER; Scripps Research, Department of Immunology and Microbiology, 10550N Torrey Pines Rd, La Jolla, CA, 92037, USA.
  • Shostak Y; Department of Immuno-Oncology, Beckman Research Institute of the City of Hope, Duarte, CA, 91010, USA.
  • Diamond DJ; Research Business Development, City of Hope, Duarte, CA, 91010, USA.
  • Wussow F; Department of Hematology and Transplant Center, City of Hope National Medical Center, Duarte, CA, 91010, USA. ddiamond@coh.org.
Nat Commun ; 11(1): 6121, 2020 11 30.
Article in English | MEDLINE | ID: covidwho-951617
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ABSTRACT
Modified Vaccinia Ankara (MVA) is a highly attenuated poxvirus vector that is widely used to develop vaccines for infectious diseases and cancer. We demonstrate the construction of a vaccine platform based on a unique three-plasmid system to efficiently generate recombinant MVA vectors from chemically synthesized DNA. In response to the ongoing global pandemic caused by SARS coronavirus-2 (SARS-CoV-2), we use this vaccine platform to rapidly produce fully synthetic MVA (sMVA) vectors co-expressing SARS-CoV-2 spike and nucleocapsid antigens, two immunodominant antigens implicated in protective immunity. We show that mice immunized with these sMVA vectors develop robust SARS-CoV-2 antigen-specific humoral and cellular immune responses, including potent neutralizing antibodies. These results demonstrate the potential of a vaccine platform based on synthetic DNA to efficiently generate recombinant MVA vectors and to rapidly develop a multi-antigenic poxvirus-based SARS-CoV-2 vaccine candidate.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Synthetic / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-19819-1

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccines, Synthetic / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / COVID-19 Vaccines Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-19819-1