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Viable SARS-CoV-2 in the air of a hospital room with COVID-19 patients.
Lednicky, John A; Lauzard, Michael; Fan, Z Hugh; Jutla, Antarpreet; Tilly, Trevor B; Gangwar, Mayank; Usmani, Moiz; Shankar, Sripriya Nannu; Mohamed, Karim; Eiguren-Fernandez, Arantza; Stephenson, Caroline J; Alam, Md Mahbubul; Elbadry, Maha A; Loeb, Julia C; Subramaniam, Kuttichantran; Waltzek, Thomas B; Cherabuddi, Kartikeya; Morris, J Glenn; Wu, Chang-Yu.
  • Lednicky JA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA; Emerging Pathogens Institute, University of Florida, USA. Electronic address: jlednicky@phhp.ufl.edu.
  • Lauzard M; Emerging Pathogens Institute, University of Florida, USA; Division of Infectious Diseases and Global Medicine, Department of Medicine, College of Medicine, University of Florida, USA.
  • Fan ZH; Department of Mechanical & Aerospace Engineering, College of Engineering, University of Florida, USA; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, USA.
  • Jutla A; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
  • Tilly TB; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
  • Gangwar M; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
  • Usmani M; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
  • Shankar SN; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
  • Mohamed K; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, USA.
  • Eiguren-Fernandez A; Aerosol Dynamics Inc., Berkeley, CA, USA.
  • Stephenson CJ; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA; Emerging Pathogens Institute, University of Florida, USA.
  • Alam MM; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA; Emerging Pathogens Institute, University of Florida, USA.
  • Elbadry MA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA; Emerging Pathogens Institute, University of Florida, USA.
  • Loeb JC; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, USA; Emerging Pathogens Institute, University of Florida, USA.
  • Subramaniam K; Emerging Pathogens Institute, University of Florida, USA; Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, USA.
  • Waltzek TB; Emerging Pathogens Institute, University of Florida, USA; Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, USA.
  • Cherabuddi K; Division of Infectious Diseases and Global Medicine, Department of Medicine, College of Medicine, University of Florida, USA.
  • Morris JG; Emerging Pathogens Institute, University of Florida, USA; Division of Infectious Diseases and Global Medicine, Department of Medicine, College of Medicine, University of Florida, USA.
  • Wu CY; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, USA.
Int J Infect Dis ; 100: 476-482, 2020 Nov.
Article in English | MEDLINE | ID: covidwho-959836
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ABSTRACT

OBJECTIVES:

Because the detection of SARS-CoV-2 RNA in aerosols but failure to isolate viable (infectious) virus are commonly reported, there is substantial controversy whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be transmitted through aerosols. This conundrum occurs because common air samplers can inactivate virions through their harsh collection processes. We sought to resolve the question whether viable SARS-CoV-2 can occur in aerosols using VIVAS air samplers that operate on a gentle water vapor condensation principle.

METHODS:

Air samples collected in the hospital room of two coronavirus disease-2019 (COVID-19) patients, one ready for discharge and the other newly admitted, were subjected to RT-qPCR and virus culture. The genomes of the SARS-CoV-2 collected from the air and isolated in cell culture were sequenced.

RESULTS:

Viable SARS-CoV-2 was isolated from air samples collected 2 to 4.8 m away from the patients. The genome sequence of the SARS-CoV-2 strain isolated from the material collected by the air samplers was identical to that isolated from the newly admitted patient. Estimates of viable viral concentrations ranged from 6 to 74 TCID50 units/L of air.

CONCLUSIONS:

Patients with respiratory manifestations of COVID-19 produce aerosols in the absence of aerosol-generating procedures that contain viable SARS-CoV-2, and these aerosols may serve as a source of transmission of the virus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Air Microbiology / Betacoronavirus Limits: Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Air Microbiology / Betacoronavirus Limits: Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2020 Document Type: Article