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Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.
Cheong, Jiyong; Yu, Hojeong; Lee, Chang Yeol; Lee, Jung-Uk; Choi, Hyun-Jung; Lee, Jae-Hyun; Lee, Hakho; Cheon, Jinwoo.
  • Cheong J; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.
  • Yu H; Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, Republic of Korea.
  • Lee CY; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.
  • Lee JU; Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, Republic of Korea.
  • Choi HJ; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Lee JH; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
  • Lee H; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.
  • Cheon J; Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, Republic of Korea.
Nat Biomed Eng ; 4(12): 1159-1167, 2020 12.
Article in English | MEDLINE | ID: covidwho-960319
ABSTRACT
The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT-qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1-2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT-qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.

Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Nat Biomed Eng Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Nat Biomed Eng Year: 2020 Document Type: Article