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Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complex.
Kato, Koki; Ikliptikawati, Dini Kurnia; Kobayashi, Akiko; Kondo, Hiroya; Lim, Keesiang; Hazawa, Masaharu; Wong, Richard W.
  • Kato K; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
  • Ikliptikawati DK; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
  • Kobayashi A; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan. Electronic address: akoba@staff.kanazawa-u.ac.jp.
  • Kondo H; Division of Transdisciplinary Sciences, Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
  • Lim K; WPI-Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan.
  • Hazawa M; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Division of Transdisciplinary Sciences
  • Wong RW; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Division of Transdisciplinary Sciences
Biochem Biophys Res Commun ; 536: 59-66, 2021 01 15.
Article in English | MEDLINE | ID: covidwho-971357
ABSTRACT
The novel human betacoronavirus SARS-CoV-2 has caused an unprecedented pandemic in the 21st century. Several studies have revealed interactions between SARS-CoV-2 viral proteins and host nucleoporins, yet their functions are largely unknown. Here, we demonstrate that the open-reading frame 6 (ORF6) of SARS-CoV-2 can directly manipulate localization and functions of nucleoporins. We found that ORF6 protein disrupted nuclear rim staining of nucleoporins RAE1 and NUP98. Consequently, this disruption caused aberrant nucleocytoplasmic trafficking and led to nuclear accumulation of mRNA transporters such as hnRNPA1. Ultimately, host cell nucleus size was reduced and cell growth was halted.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Proteins / Nucleocytoplasmic Transport Proteins / Nuclear Pore Complex Proteins / Nuclear Matrix-Associated Proteins / Cell Nucleus Size Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2020.11.115

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Proteins / Nucleocytoplasmic Transport Proteins / Nuclear Pore Complex Proteins / Nuclear Matrix-Associated Proteins / Cell Nucleus Size Limits: Humans Language: English Journal: Biochem Biophys Res Commun Year: 2021 Document Type: Article Affiliation country: J.bbrc.2020.11.115