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The kinetics of humoral response and its relationship with the disease severity in COVID-19.
Ren, Lili; Zhang, Lulu; Chang, De; Wang, Junwen; Hu, Yongfeng; Chen, Hong; Guo, Li; Wu, Chao; Wang, Conghui; Wang, Yingying; Wang, Ying; Wang, Geng; Yang, Siyuan; Dela Cruz, Charles S; Sharma, Lokesh; Wang, Linghang; Zhang, Dingyu; Wang, Jianwei.
  • Ren L; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Zhang L; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, P.R. China.
  • Chang; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wang J; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, P.R. China.
  • Hu Y; Third Medical Center of Chinese PLA General Hospital, Beijing, 100039, P.R. China.
  • Chen H; Wuhan 4th hospital, Wuhan, 430023, P.R. China.
  • Guo L; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wu C; The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, P.R. China.
  • Wang C; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wang Y; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wang Y; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wang G; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Yang S; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Dela Cruz CS; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Sharma L; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, P.R. China.
  • Wang L; Laboratory of Infectious Diseases Center of Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, P.R. China.
  • Zhang D; Section of Pulmonary and Critical Care and Sleep Medicine, Department of Medicine, Yale University School of Medicine, New Haven, CT, 06520, USA.
  • Wang J; Section of Pulmonary and Critical Care and Sleep Medicine, Department of Medicine, Yale University School of Medicine, New Haven, CT, 06520, USA. lokeshkumar.sharma@yale.edu.
Commun Biol ; 3(1): 780, 2020 12 11.
Article in English | MEDLINE | ID: covidwho-975030
ABSTRACT
Coronavirus Disease 2019 (COVID-19) has caused a global pandemic. Here we profiled the humoral response against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by measuring immunoglobulin (Ig) A, IgM, and IgG against nucleocapsid and spike proteins, along with IgM and IgG antibodies against receptor-binding domain (RBD) of the spike protein and total neutralizing antibodies (NAbs). We tested 279 plasma samples collected from 176 COVID-19 patients who presented and enrolled at different stages of their disease. Plasma dilutions were optimized and based on the data, a single dilution of plasma was used. The mean absorbance at 450 nm was measured for Ig levels and NAbs were measured using geometric mean titers. We demonstrate that more severe cases have a late-onset in the humoral response compared to mild/moderate infections. All the antibody titers continue to rise in patients with COVID-19 over the disease course. However, these levels are mostly unrelated to disease severity. The appearance time and titers of NAbs showed a significant positive correlation to the antibodies against spike protein. Our results suggest the late onset of antibody response as a risk factor for disease severity, however, there is a limited role of antibody titers in predicting disease severity of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Immunity, Humoral / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: Commun Biol Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Immunity, Humoral / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adolescent / Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Country/Region as subject: Asia Language: English Journal: Commun Biol Year: 2020 Document Type: Article