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Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays.
Zava, Theodore T; Zava, David T.
  • Zava TT; ZRT Laboratory, 8605 SW Creekside Pl., Beaverton, OR 97008, USA.
  • Zava DT; ZRT Laboratory, 8605 SW Creekside Pl., Beaverton, OR 97008, USA.
Bioanalysis ; 13(1): 13-28, 2021 Jan.
Article in English | MEDLINE | ID: covidwho-977787
Semantic information from SemMedBD (by NLM)
1. Immunoassay method USES immunoglobulin G
Subject
Immunoassay method
Predicate
USES
Object
immunoglobulin G
2. Assay USES Protein Subunits
Subject
Assay
Predicate
USES
Object
Protein Subunits
3. Assay USES Nucleocapsid
Subject
Assay
Predicate
USES
Object
Nucleocapsid
4. Assay USES immunoglobulin G
Subject
Assay
Predicate
USES
Object
immunoglobulin G
5. Immunoassay method USES immunoglobulin G
Subject
Immunoassay method
Predicate
USES
Object
immunoglobulin G
6. Assay USES Protein Subunits
Subject
Assay
Predicate
USES
Object
Protein Subunits
7. Assay USES Nucleocapsid
Subject
Assay
Predicate
USES
Object
Nucleocapsid
8. Assay USES immunoglobulin G
Subject
Assay
Predicate
USES
Object
immunoglobulin G
ABSTRACT

Aim:

Coronavirus disease 2019 antibody testing often relies on venous blood collection, which is labor-intensive, inconvenient and expensive compared with finger-stick capillary dried blood spot (DBS) collection. The purpose of our work was to determine if two commercially available anti-severe acute respiratory syndrome coronavirus 2 enzyme-linked immunosorbent assays for IgG antibodies against spike S1 subunit and nucleocapsid proteins could be validated for use with DBS. Materials &

methods:

Kit supplied reagents were used to extract DBS, and in-house DBS calibrators were included on every run.

Results:

Positive/negative concordance between DBS and serum was 100/99.3% for the spike S1 subunit assay and 100/98% for the nucleocapsid assay.

Conclusion:

Validation of the DBS Coronavirus disease 2019 IgG antibody assays demonstrated that serum and DBS can produce equivalent results with minimal kit modifications.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Enzyme-Linked Immunosorbent Assay / Dried Blood Spot Testing / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: Bioanalysis Year: 2021 Document Type: Article Affiliation country: Bio-2020-0289

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Enzyme-Linked Immunosorbent Assay / Dried Blood Spot Testing / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: Bioanalysis Year: 2021 Document Type: Article Affiliation country: Bio-2020-0289