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Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus.
Orlova, O V; Glazkova, D V; Tsyganova, G M; Antoshkina, I V; Mintaev, R R; Tikhonov, A S; Bogoslovskaya, E V; Shipulin, G A.
  • Orlova OV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia. Electronic address: orlovaov@gmail.com.
  • Glazkova DV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
  • Tsyganova GM; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
  • Antoshkina IV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
  • Mintaev RR; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia; I. Mechnikov Research Institute of Vaccines and Sera, 105064, Moscow, Russia.
  • Tikhonov AS; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
  • Bogoslovskaya EV; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
  • Shipulin GA; Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks¼ of the Federal Medical Biological Agency", 119121, Moscow, Russia.
J Virol Methods ; 289: 114056, 2021 03.
Article in English | MEDLINE | ID: covidwho-988687
ABSTRACT
Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccinia virus / Genetic Vectors / SARS-CoV-2 / Antigens, Viral Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Virol Methods Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Vaccinia virus / Genetic Vectors / SARS-CoV-2 / Antigens, Viral Topics: Vaccines Limits: Animals / Humans Language: English Journal: J Virol Methods Year: 2021 Document Type: Article