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Expression of Ace2, Tmprss2, and Furin in mouse ear tissue
Tsukasa Uranaka; Akinori Kashio; Rumi Ueha; Taku Sato; Bing Han; Ying Gao; Makoto Kinoshita; Kenji Kondo; Tatsuya Yamasoba.
  • Tsukasa Uranaka; The University of Tokyo
  • Akinori Kashio; The University of Tokyo
  • Rumi Ueha; The University of Tokyo
  • Taku Sato; The University of Tokyo
  • Bing Han; The University of Tokyo
  • Ying Gao; The University of Tokyo
  • Makoto Kinoshita; The University of Tokyo
  • Kenji Kondo; The University of Tokyo
  • Tatsuya Yamasoba; The University of Tokyo
Preprint in English | bioRxiv | ID: ppbiorxiv-164335
ABSTRACT
ObjectivesIntracellular entry of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) depends on the interaction between its spike protein to a cellular receptor named angiotensin-converting enzyme 2 (ACE2) and depends on Furin-mediated spike 23 protein cleavage and spike protein priming by host cell proteases including 24 transmembrane protease serine 2 (TMPRSS2). Tmprss1, Tmprss3, and Tmprss5 are expressed in the spiral ganglion neurons and the organ of Corti in the inner ear; however, Ace2, Tmprss2, and Furin expression profiles in the middle ear remain unclear. Therefore, this study aimed to analyze Ace2, Tmprss2, and Furin expression in the middle and inner ear of mice. Study DesignAnimal research. SettingDepartment of Otolaryngology and Head and Neck Surgery, University of Tokyo. MethodsWe performed immunohistochemical analysis to examine the distribution of Ace2, Tmprss2, and Furin in the eustachian tube, middle ear space, and cochlea of mice. ResultsAce2 was expressed in the cytoplasm in the middle ear epithelium, eustachian tube epithelium, stria vascularis, and spiral ganglion. Tmprss2 and Furin were widely expressed in the middle ear spaces and the cochlea. ConclusionCo-expression of Ace2, Tmprss2, and Furin in the middle ear indicates that the middle ear is susceptible to SARS-CoV-2 infections, thus warranting the use of personal protective equipment during mastoidectomy for coronavirus disease (COVID-19) patients.
Full text: Available Collection: Preprints Database: bioRxiv Document Type: Preprint Language: English Year: 2020

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Full text: Available Collection: Preprints Database: bioRxiv Document Type: Preprint Language: English Year: 2020
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