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How early into the outbreak can surveillance of SARS-CoV-2 in wastewater tell us?
Peiying Hong; Andri Taruna Rachmadi; David Mantilla-Calderon; Mohsen Alkahtani; Yasir M. Bashwari; Hamed Al Qarni; Jianqiang Zhou.
  • Peiying Hong; King Abdullah University of Science and Technology
  • Andri Taruna Rachmadi; King Abdullah University of Science and Technology
  • David Mantilla-Calderon; King Abdullah University of Science and Technology
  • Mohsen Alkahtani; Ministry of Health
  • Yasir M. Bashwari; Ministry of Health
  • Hamed Al Qarni; Ministry of Health
  • Jianqiang Zhou; King Abdullah University of Science and Technology
Preprint in English | medRxiv | ID: ppmedrxiv-20177667
ABSTRACT
There is increasing interest to use wastewater-based surveillance of SARS-CoV-2 as an early warning of the outbreak within a community. Despite successful detection of SARS-CoV-2 in wastewaters sampled from multiple locations, there is still no clear idea on the minimal number of cases needed in a community to result in a positive detection of the virus in wastewaters. To address this knowledge gap, we sampled wastewaters from a septic tank and biological activated sludge tank located on-site of a hospital. The hospital is providing treatment for SARS-CoV-2 infected patients, with the number of hospitalized patients per day known. It was observed that > 253 positive cases out of 10,000 persons are required prior to detecting SARS-CoV-2 in wastewater. There was a weak correlation between N1 and N2 gene abundances in wastewater with the number of hospitalized cases. This correlation was however not observed for N3 gene. The occurrence frequency of SARS-CoV-2 is at least 5 times lower in the partially treated wastewater than in the septic tank. Furthermore, abundance of N1 and N3 genes in the activated sludge tank were 50 and 70% of the levels detected in septic tank, suggesting poor persistence of the SARS-CoV-2 gene fragments in wastewater. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=115 SRC="FIGDIR/small/20177667v1_ufig1.gif" ALT="Figure 1"> View larger version (28K) org.highwire.dtl.DTLVardef@3c868eorg.highwire.dtl.DTLVardef@1a5ec59org.highwire.dtl.DTLVardef@3fcafborg.highwire.dtl.DTLVardef@273440_HPS_FORMAT_FIGEXP M_FIG C_FIG
Full text: Available Collection: Preprints Database: medRxiv Language: English Year: 2020 Document Type: Preprint

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Full text: Available Collection: Preprints Database: medRxiv Language: English Year: 2020 Document Type: Preprint