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Longitudinal detection of SARS-CoV-2-specific antibody responses with different serological methods
Petra Emmerich; Ronald von Possel; Christoph Josef Hemmer; Carlos Fritzsche; Hilte Geerdes-Fenge; Babett Menge; Claudia Messing; Viola Borchardt-Lohoelter; Christina Deschermeier; Katja Steinhagen.
  • Petra Emmerich; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; Department of Tropical Medicine and Infectious Diseases, University of
  • Ronald von Possel; Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; Department of Tropical Medicine and Infectious Diseases, University of
  • Christoph Josef Hemmer; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany
  • Carlos Fritzsche; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany
  • Hilte Geerdes-Fenge; Department of Tropical Medicine and Infectious Diseases, Center for Internal Medicine, University of Rostock, Rostock, Germany
  • Babett Menge; Institute for Experimental Immunology, affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany
  • Claudia Messing; Institute for Experimental Immunology, affiliated to EUROIMMUN AG
  • Viola Borchardt-Lohoelter; Institute for Experimental Immunology, affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany
  • Christina Deschermeier; Department for Infectious Disease Diagnostics, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
  • Katja Steinhagen; Institute for Experimental Immunology, affiliated with EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany
Preprint in English | medRxiv | ID: ppmedrxiv-21255608
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ABSTRACT
Serological testing for anti-SARS-CoV-2 antibodies is used to detect ongoing or past SARS-CoV-2 infections. To study the kinetics of anti-SARS-CoV-2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with PCR-confirmed COVID-19 and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of IgG, IgM and IgA antibodies using indirect immunofluorescence testing (IIFT) based on SARS-CoV-2-infected cells. They were further tested for antibodies against the S1 domain of the SARS-CoV-2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using ELISA. The assay specificities were 94.4%-100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed highest sensitivities due to the use of the whole SARS-CoV-2 as substrate and provided information whether or not the individual has been infected with SARS-CoV-2. ELISAs provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS-CoV-2.
Full text: Available Collection: Preprints Database: medRxiv Document Type: Preprint Language: English Year: 2021

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Full text: Available Collection: Preprints Database: medRxiv Document Type: Preprint Language: English Year: 2021
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