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Existence of blood circulating immune-cell clusters (CICs) comprising antigen-presenting cells and B cells (preprint)
biorxiv; 2022.
Preprint
in English
| bioRxiv | ID: ppzbmed-10.1101.2022.01.26.477834
ABSTRACT
Cell-to-cell physical interactions are involved in almost every physiological processes in multicellular organisms. Although the dynamics of these interactions could be highly diverse and complex in many circumstances, certain cell-to-cell interactions among immune cells have been well studied due importance in understanding disease pathogenesis and immune therapy development 1 . Dendritic cells (DCs) and B cells are directly involved in adaptive immune response against pathogens. Interaction mechanism between these two celltypes is well-known to occur in germinal centers either indirectly via helper T (Th) cells or directly via cell contact. However, there are animal in vitro and in vivo evidence that such direct DC-to-B cell contact can occur outside germinal centers like in peripheral blood or collagen matrix and display antiviral immune-related activity 2,3 . Here, we provide evidence that certain types of antigen presenting cells (APCs) can form robust cell clusters with B cells and circulate in blood. From healthy human blood immune single cell RNA-seq datasets, we detected APC subpopulations (0.34±0.19% of total PBMCs) that were also enriched with well-known naïve B cell markers. We visually observed DCB doublets and multiplets (∼0.69% of total live PBMCs) in wildtype mouse blood using flow cytometry and microscopic imaging, thus proving the existence of circulating immune-cell clusters (CICs) composed of APCs and B cells. BCR repertoire of these healthy mouse CICs were similar to circulating B cells. Noticeably, frequency of these APCB CICs were higher COVID-19 patients than healthy donors and their B cell subtype composition (e.g. naïve, plasmablast, IgM + , IgG + ) varied with disease severity.
Full text:
Available
Collection:
Preprints
Database:
bioRxiv
Main subject:
Adenomatous Polyposis Coli
/
COVID-19
Language:
English
Year:
2022
Document Type:
Preprint
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