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Atomically accurate de novo design of single-domain antibodies (preprint)
biorxiv; 2024.
Preprint
in English
| bioRxiv | ID: ppzbmed-10.1101.2024.03.14.585103
ABSTRACT
Despite the central role that antibodies play in modern medicine, there is currently no way to rationally design novel antibodies to bind a specific epitope on a target. Instead, antibody discovery currently involves time-consuming immunization of an animal or library screening approaches. Here we demonstrate that a fine-tuned RFdiffusion network is capable of designing de novo antibody variable heavy chains (VHH's) that bind user-specified epitopes. We experimentally confirm binders to four disease-relevant epitopes, and the cryo-EM structure of a designed VHH bound to influenza hemagglutinin is nearly identical to the design model both in the configuration of the CDR loops and the overall binding pose.
Full text:
Available
Collection:
Preprints
Database:
bioRxiv
Main subject:
Heavy Chain Disease
Language:
English
Year:
2024
Document Type:
Preprint
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