This article is a Preprint
Preprints are preliminary research reports that have not been certified by peer review. They should not be relied on to guide clinical practice or health-related behavior and should not be reported in news media as established information.
Preprints posted online allow authors to receive rapid feedback and the entire scientific community can appraise the work for themselves and respond appropriately. Those comments are posted alongside the preprints for anyone to read them and serve as a post publication assessment.
Polymerized Porin as a Novel Delivery Platform for Coronavirus Vaccine (preprint)
researchsquare; 2021.
Preprint
in English
| PREPRINT-RESEARCHSQUARE | ID: ppzbmed-10.21203.rs.3.rs-1128338.v1
ABSTRACT
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), seriously threatens human life and health. The correct folding and polymerization of the receptor-binding domain (RBD) protein of coronavirus in Escherichia coli may reduce the cost of SARS-CoV-2 vaccines. Here, we designed this nanopore by using the principle of ClyA porin polymerization triggered by the cell membrane. We use surfactants to "pick" the ClyA-RBD nanopore from the bacterial outer membrane in this study. More importantly, the polymerized RBD displayed on ClyA-RBD polymerized porin (RBD-PP) already has some correct spatial structures of virus spikes. The nanostructures of RBD-PP can target lymph nodes and promote antigen uptake and processing by dendritic cells, thereby effectively eliciting the production of anti-SARS-CoV-2 neutralizing antibodies and systemic cellular immune responses and immune memory. We applied ofthis PP-based vaccine platform to make an RBD-based subunit vaccine against SARS-CoV-2, which will provide a foundation for the development of inexpensive coronavirus vaccines. The development of novel vaccine delivery system is an important part of innovative drug research. This novel PP-based vaccine platform is likely to be applied to more fields, including other viral vaccines, bacterial vaccines, tumor vaccines, drug delivery, and disease diagnosis.
Full text:
Available
Collection:
Preprints
Database:
PREPRINT-RESEARCHSQUARE
Main subject:
COVID-19
Language:
English
Year:
2021
Document Type:
Preprint
Similar
MEDLINE
...
LILACS
LIS