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Erythrocytes Induce Vascular Dysfunction in COVID-19 (preprint)
ssrn; 2021.
Preprint
in English
| PREPRINT-SSRN | ID: ppzbmed-10.2139.ssrn.3945298
ABSTRACT
Background:
Vascular injury has been implicated as a major cause of clinical complications in patients with coronavirus disease 2019 (COVID-19) based on autopsy studies showing destruction of the endothelial architecture. Red blood cells (RBCs) are affected by COVID-19 with alterations in their structure and function, possibly altering disease progress.Objectives:
This study was designed to test the hypothesis of persistent endothelial dysfunction and that RBCs act as mediators of endothelial dysfunction in COVID-19. Methods andresults:
COVID-19 patients displayed profound endothelial dysfunction in vivo assessed with pulse amplitude tonometry, both in the acute phase and at follow-up four months later. RBCs but not plasma from COVID-19 patients in the acute phase incubated with healthy rat aorta induced severe endothelial dysfunction ex vivo compared to RBCs from healthy subjects. Further, these RBCs induced vascular arginase 1 and oxidative stress. Consequently, inhibition of vascular arginase or superoxide attenuated endothelial dysfunction induced by RBCs from COVID-19 patients. These RBCs were characterized by increased production of reactive oxygen species and reduced export of the nitric oxide metabolite nitrate. RBCs from COVID-19 patients at follow-up did not affect vascular function. Pre-incubation of RBCs from healthy subjects with interferon-γ impaired endothelial function.Conclusions:
This study demonstrates the presence of persistent endothelial dysfunction in an otherwise mainly healthy population hospitalized for COVID-19, and implicates a role of RBCs as mediators of endothelial injury. These data shed light on a new pathological mechanism underlying vascular dysfunction in COVID-19 and lay the foundation for future therapeutic developments.
Full text:
Available
Collection:
Preprints
Database:
PREPRINT-SSRN
Main subject:
Wounds and Injuries
/
Vascular Neoplasms
/
Hyperargininemia
/
Iridocorneal Endothelial Syndrome
/
Vascular System Injuries
/
COVID-19
Language:
English
Year:
2021
Document Type:
Preprint
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