A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores.
Biosens Bioelectron
; 177: 113005, 2021 Apr 01.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1033431
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Hidrogeles
/
Ácidos Nucleicos Inmovilizados
/
Pruebas en el Punto de Atención
/
Prueba de Ácido Nucleico para COVID-19
/
SARS-CoV-2
/
COVID-19
Tipo de estudio:
Estudios diagnósticos
Límite:
Humanos
Idioma:
Inglés
Revista:
Biosens Bioelectron
Asunto de la revista:
Biotecnologia
Año:
2021
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS