The porcine deltacoronavirus accessory protein NS6 is expressed in vivo and incorporated into virions.
Virology
; 556: 1-8, 2021 04.
Artículo
en Inglés
| MEDLINE | ID: covidwho-1045103
ABSTRACT
Porcine deltacoronavirus (PDCoV) is one of the emerged coronaviruses posing a significant threat to the swine industry. Previous work showed the presence of a viral accessory protein NS6 in PDCoV-infected cells. In this study, we detected the expression of the NS6 protein in small intestinal tissues of PDCoV-infected piglets. In addition, SDS-PAGE and Western blot analysis of sucrose gradient-purified virions showed the presence of a 13-kDa NS6 protein. Further evidences of the presence of NS6 in the PDCoV virions were obtained by immunogold staining of purified virions with anti-NS6 antiserum, and by immunoprecipitation of NS6 from purified virions. Finally, the anti-NS6 antibody was not able to neutralize PDCoV in cultured cells. These data establish for the first time that the accessory protein NS6 is expressed during infection in vivo and incorporated into PDCoV virions.
Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
/
Virión
/
Proteínas no Estructurales Virales
/
Infecciones por Coronavirus
/
Deltacoronavirus
Límite:
Animales
Idioma:
Inglés
Revista:
Virology
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
J.virol.2021.01.011
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