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LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community.
Moehling, Taylor J; Choi, Gihoon; Dugan, Lawrence C; Salit, Marc; Meagher, Robert J.
  • Moehling TJ; Sandia National Laboratories, Biotechnology & Bioengineering Dept., Livermore, CA, USA.
  • Choi G; Sandia National Laboratories, Biotechnology & Bioengineering Dept., Livermore, CA, USA.
  • Dugan LC; Lawrence Livermore National Laboratory, Biosciences & Biotechnology Div., Livermore, CA, USA.
  • Salit M; Joint Initiative for Metrology in Biology, SLAC National Accelerator Lab and Departments of Bioengineering and Pathology, Stanford University, Stanford, CA, USA.
  • Meagher RJ; Sandia National Laboratories, Biotechnology & Bioengineering Dept., Livermore, CA, USA.
Expert Rev Mol Diagn ; 21(1): 43-61, 2021 01.
Artículo en Inglés | MEDLINE | ID: covidwho-1066147
ABSTRACT

Introduction:

Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions.Areas covered In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting.Expert opinion Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization.
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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular / Pruebas en el Punto de Atención / Prueba de COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Expert Rev Mol Diagn Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo País de afiliación: 14737159.2021.1873769

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Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico / Técnicas de Diagnóstico Molecular / Pruebas en el Punto de Atención / Prueba de COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Estudios diagnósticos / Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Expert Rev Mol Diagn Asunto de la revista: Biologia Molecular Año: 2021 Tipo del documento: Artículo País de afiliación: 14737159.2021.1873769