Your browser doesn't support javascript.
Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents.
Haile, Simon; Nikiforuk, Aidan M; Pandoh, Pawan K; Twa, David D W; Smailus, Duane E; Nguyen, Jason; Pleasance, Stephen; Wong, Angus; Zhao, Yongjun; Eisler, Diane; Moksa, Michelle; Cao, Qi; Wong, Marcus; Su, Edmund; Krzywinski, Martin; Nelson, Jessica; Mungall, Andrew J; Tsang, Frankie; Prentice, Leah M; Jassem, Agatha; Manges, Amee R; Jones, Steven J M; Coope, Robin J; Prystajecky, Natalie; Marra, Marco A; Krajden, Mel; Hirst, Martin.
  • Haile S; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nikiforuk AM; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada.
  • Pandoh PK; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Twa DDW; BC Cancer Research Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Smailus DE; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nguyen J; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Pleasance S; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Wong A; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Zhao Y; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Eisler D; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Moksa M; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Cao Q; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Wong M; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Su E; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Krzywinski M; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nelson J; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Mungall AJ; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Tsang F; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Prentice LM; Provincial Laboratory Medicine Services, Provincial Health Services Authority, Vancouver, British Columbia, Canada.
  • Jassem A; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Manges AR; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Jones SJM; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
  • Coope RJ; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Prystajecky N; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Marra MA; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
  • Krajden M; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Hirst M; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada. Electronic address: hirstm@mail.ubc.ca.
J Virol Methods ; 299: 114339, 2022 01.
Artículo en Inglés | MEDLINE | ID: covidwho-1472084
Preprint
Este artículo de revista científica es probablemente basado en un preprint previamente disponible, por medio del reconocimiento de similitud realizado por una máquina. La confirmación humana aún está pendiente.
Ver preprint
ABSTRACT
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / COVID-19 Tipo de estudio: Estudios diagnósticos / Ensayo controlado aleatorizado Límite: Humanos Idioma: Inglés Revista: J Virol Methods Año: 2022 Tipo del documento: Artículo País de afiliación: J.jviromet.2021.114339

Similares

MEDLINE
LILACS
LIS
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: Disponible Colección: Bases de datos internacionales Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / COVID-19 Tipo de estudio: Estudios diagnósticos / Ensayo controlado aleatorizado Límite: Humanos Idioma: Inglés Revista: J Virol Methods Año: 2022 Tipo del documento: Artículo País de afiliación: J.jviromet.2021.114339