Protocol design for the evaluation of chronic antioxidant supplementation on oxidative stress and cognition status in post COVID-19 patients

ABSTRACT

Post COVID-19 sequelae include several complications including cognitive impairment, a situation associated with increased oxidative stress. In a pilot study, we intend to recruit 20 patients having a MOCA (MOntreal Cognitive Assessment questionnaire) score ≤ 25 after their discharge from hospital for COVID-19 infection requiring a long stay (> 30 days) in Intensive Care Unit. The goal of our study was to check how a blend of polyphenols (French Grape (Vitis vinifera L.) and North-American Wild Blueberry (Vaccinium angustifolium A) extracts)) at 800 mg enriched with vitamins and minerals at nutritional doses (Memophenol™l) and given each day during 6 months could potentially decrease oxidative stress and improve cognitive status when compared to a placebo. For that, we propose to investigate a large battery of tests including the determination of antioxidants (vitamins C and E (alpha- and gamma-tocopherol), beta-carotene, glutathione, thiol proteins, total polyphenols, paraoxonase, glutathione peroxidase), trace elements (copper, zinc, selenium), oxidative damages to lipids (lipid peroxides, oxidized LDL) and inflammatory biomarkers (myeloperoxidase), respectively before supplementation, 3 and 6 months after. In parallel, the evolution of the MOCA score will be followed. Actually 8 patients have been included in the study.