Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells.
Immun Inflamm Dis
; 8(4): 753-762, 2020 12.
Artículo
en Inglés
| MEDLINE | ID: covidwho-893223
ABSTRACT
BACKGROUND:
Severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) is a single-stranded RNA virus responsible for the global pandemic of the coronavirus disease-2019 (COVID-19). To date, there are still no effective approaches for the prevention and treatment of COVID-19.OBJECTIVE:
The present study aims to explore the possible mechanisms of SARS-CoV-2 infection in human lung cells.METHODS:
Data interpretation was conducted by recruiting bioinformatics analysis, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways analysis using downloaded data from the NCBI Gene Expression Omnibus database.RESULTS:
The present study demonstrated that SARS-CoV-2 infection induces the upregulation of 14 interferon-stimulated genes, indicative of immune, and interferon responses to the virus. Notably, genes for pyrimidine metabolism and steroid hormone biosynthesis are selectively enriched in human lung cells after SARS-CoV-2 infection, suggesting that altered pyrimidine metabolism and steroid biosynthesis are remarkable, and perhaps druggable features after SARS-CoV-2 infection. Besides, there is a strong positive correlation between viral ORF1ab, ORF6, and angiotensin-converting enzyme 2 (ACE2) expression in human lung cells, implying that ACE2 facilitates SARS-CoV-2 infection and replication in host cells probably through the induction of ORF1ab and ORF6.Palabras clave
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
MEDLINE
Asunto principal:
Neumonía Viral
/
Interferones
/
Infecciones por Coronavirus
/
Peptidil-Dipeptidasa A
/
Betacoronavirus
/
Pulmón
Límite:
Humanos
Idioma:
Inglés
Revista:
Immun Inflamm Dis
Año:
2020
Tipo del documento:
Artículo
País de afiliación:
Iid3.366
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