Molecular diagnostic assays for COVID-19: an overview.
Crit Rev Clin Lab Sci
; 58(6): 385-398, 2021 09.
Article
Dans Anglais
| MEDLINE | ID: covidwho-1087600
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the cardinal importance of rapid and accurate diagnostic assays. Since the early days of the outbreak, researchers with different scientific backgrounds across the globe have tried to fulfill the urgent need for such assays, with many assays having been approved and with others still undergoing clinical validation. Molecular diagnostic assays are a major group of tests used to diagnose COVID-19. Currently, the detection of SARS-CoV-2 RNA by reverse transcription polymerase chain reaction (RT-PCR) is the most widely used method. Other diagnostic molecular methods, including CRISPR-based assays, isothermal nucleic acid amplification methods, digital PCR, microarray assays, and next generation sequencing (NGS), are promising alternatives. In this review, we summarize the technical and clinical applications of the different COVID-19 molecular diagnostic assays and suggest directions for the implementation of such technologies in future infectious disease outbreaks.
Mots clés
Texte intégral:
Disponible
Collection:
Bases de données internationales
Base de données:
MEDLINE
Sujet Principal:
Techniques d'amplification d'acides nucléiques
/
Techniques de diagnostic moléculaire
/
SARS-CoV-2
/
COVID-19
Type d'étude:
Étude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Revue:
Crit Rev Clin Lab Sci
Thème du journal:
Techniques et procédures de laboratoire
Année:
2021
Type de document:
Article
Pays d'affiliation:
10408363.2021.1884640
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