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Antibody Responses to BNT162b2 Vaccination in Japan: Monitoring Vaccine Efficacy by Measuring IgG Antibodies against the Receptor-Binding Domain of SARS-CoV-2.
Fujigaki, Hidetsugu; Yamamoto, Yasuko; Koseki, Takenao; Banno, Sumi; Ando, Tatsuya; Ito, Hiroyasu; Fujita, Takashi; Naruse, Hiroyuki; Hata, Tadayoshi; Moriyama, Saya; Takahashi, Yoshimasa; Suzuki, Tadaki; Murakami, Takahiro; Yoshida, Yukihiro; Yagura, Yo; Oyamada, Takayoshi; Takemura, Masao; Kondo, Masashi; Iwata, Mitsunaga; Saito, Kuniaki.
  • Fujigaki H; Department of Advanced Diagnostic System Development, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, Japan.
  • Yamamoto Y; Department of Advanced Diagnostic System Development, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, Japan.
  • Koseki T; Department of Clinical Pharmacy, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Banno S; Center for Clinical Trial and Research Support, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Ando T; Center for Clinical Trial and Research Support, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Ito H; Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Fujita T; Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Naruse H; Department of Clinical Laboratory, Fujita Health University Hospital, Toyoake, Aichi, Japan.
  • Hata T; Department of Medical Laboratory Science, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, Japan.
  • Moriyama S; Department of Medical Laboratory Science, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, Japan.
  • Takahashi Y; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, Japan.
  • Suzuki T; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Tokyo, Japan.
  • Murakami T; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
  • Yoshida Y; Diagnostics Research Laboratories, Diagnostics Technical Service & Research Operations, Diagnostics Division, FUJIFILM Wako Pure Chemical Corporation, Amagasaki, Hyogo, Japan.
  • Yagura Y; Diagnostics Research Laboratories, Diagnostics Technical Service & Research Operations, Diagnostics Division, FUJIFILM Wako Pure Chemical Corporation, Amagasaki, Hyogo, Japan.
  • Oyamada T; Diagnostics Research Laboratories, Diagnostics Technical Service & Research Operations, Diagnostics Division, FUJIFILM Wako Pure Chemical Corporation, Amagasaki, Hyogo, Japan.
  • Takemura M; In Vitro Diagnostics Division, FUJIFILM Corporation, Tokyo, Japan.
  • Kondo M; Department of Advanced Diagnostic System Development, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, Japan.
  • Iwata M; Center for Clinical Trial and Research Support, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
  • Saito K; Department of Respiratory Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Microbiol Spectr ; 10(1): e0118121, 2022 02 23.
Article Dans Anglais | MEDLINE | ID: covidwho-1632412
Preprint
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ABSTRACT
To fight severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), mass vaccination has begun in many countries. To investigate the usefulness of a serological assay to predict vaccine efficacy, we analyzed the levels of IgG, IgM, and IgA against the receptor-binding domain (RBD) of SARS-CoV-2 in the sera from BNT162b2 vaccinated individuals in Japan. This study included 219 individuals who received two doses of BNT162b2. The levels of IgG, IgM, and IgA against RBD were measured by enzyme-linked immunosorbent assay before and after the first and second vaccination, respectively. The relationship between antibody levels and several factors, including age, gender, and hypertension were analyzed. Virus-neutralizing activity in sera was measured to determine the correlation with the levels of antibodies. A chemiluminescent enzyme immunoassay (CLEIA) method to measure IgG against RBD was developed and validated for the clinical setting. The levels of all antibody isotypes were increased after vaccination. Among them, RBD-IgG was dramatically increased after the second vaccination. The IgG levels in females were significantly higher than in males. There was a negative correlation between age and IgG levels in males. The IgG levels significantly correlated with the neutralizing activity. The CLEIA assay measuring IgG against RBD showed a reliable performance and a high correlation with neutralizing activity. Monitoring of IgG against RBD is a powerful tool to predict the efficacy of SARS-CoV-2 vaccination and provides useful information in considering a personalized vaccination strategy for COVID-19. IMPORTANCE Mass vaccination campaigns using mRNA vaccines against SARS-CoV-2 have begun in many countries. Serological assays to detect antibody production may be a useful tool to monitor the efficacy of SARS-CoV-2 vaccination in individuals. Here, we reported the induction of antibody isotype responses after the first and second dose of the BNT162b2 vaccine in a well-defined cohort of employees in Japan. We also reported that age, gender, and hypertension are associated with differences in antibody response after vaccination. This study not only provides valuable information with respect to antibody responses after BNT162b2 vaccination in the Japanese population but also the usefulness of serological assays for monitoring vaccine efficacy in clinical laboratories to determine a personalized vaccination strategy for COVID-19.
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Texte intégral: Disponible Collection: Bases de données internationales Base de données: MEDLINE Sujet Principal: Vaccins contre la COVID-19 / SARS-CoV-2 / Anticorps antiviraux Type d'étude: Étude de cohorte / Études expérimentales / Étude observationnelle / Étude pronostique Les sujets: Vaccins Limites du sujet: Adulte / Femelle / Humains / Mâle / Adulte d'âge moyen / Jeune adulte Pays comme sujet: Asie langue: Anglais Revue: Microbiol Spectr Année: 2022 Type de document: Article Pays d'affiliation: Spectrum.01181-21

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Texte intégral: Disponible Collection: Bases de données internationales Base de données: MEDLINE Sujet Principal: Vaccins contre la COVID-19 / SARS-CoV-2 / Anticorps antiviraux Type d'étude: Étude de cohorte / Études expérimentales / Étude observationnelle / Étude pronostique Les sujets: Vaccins Limites du sujet: Adulte / Femelle / Humains / Mâle / Adulte d'âge moyen / Jeune adulte Pays comme sujet: Asie langue: Anglais Revue: Microbiol Spectr Année: 2022 Type de document: Article Pays d'affiliation: Spectrum.01181-21