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Development and Implementation of Dried Blood Spot-based COVID-19 Serological Assays for Epidemiologic Studies
Marcus P Wong; Michelle Meas; Cameron Adams; Samantha Hernandez; Valerie Green; Magelda Montoya; Brett M Hirsch; Mary Horton; Hong Quach; Diana Quach; Xiaorong Shao; Indro Fedrigo; Alexandria Zermeno; Julia Huffaker; Raymond Montes; Alicia Madden; Sherri Cyrus; David McDowell; Phillip Williamson; Paul Contestable; Mars Stone; Josefina Coloma; Michael Paul Busch; Lisa Barcellos; Eva Harris.
  • Marcus P Wong; University of California, Berkeley, School of Public Health
  • Michelle Meas; University of California Berkeley, School of Public Health
  • Cameron Adams; University of California, Berkeley, School of Public Health
  • Samantha Hernandez; University of California, Berkeley, School of Public Health
  • Valerie Green; Creative Testing Solutions
  • Magelda Montoya; University of California, Berkeley, School of Public Health
  • Brett M Hirsch; Ortho Clinical Diagnostics
  • Mary Horton; University of California, Berkeley, School of Public Health
  • Hong Quach; University of California, Berkeley, School of Public Health
  • Diana Quach; University of California, Berkeley, School of Public Health
  • Xiaorong Shao; University of California, Berkeley, School of Public Health
  • Indro Fedrigo; University of California, Berkeley, School of Public Health
  • Alexandria Zermeno; University of California, Berkeley, School of Public Health
  • Julia Huffaker; University of California, Berkeley, School of Public Health
  • Raymond Montes; University of California, Berkeley, School of Public Health
  • Alicia Madden; University of California, Berkeley, School of Public Health
  • Sherri Cyrus; Creative Testing Solutions
  • David McDowell; Creative Testing Solutions
  • Phillip Williamson; Creative Testing Solutions
  • Paul Contestable; Ortho Clinical Diagnostics
  • Mars Stone; Vitalant Research Institiute
  • Josefina Coloma; University of California, Berkeley, School of Public Health
  • Michael Paul Busch; VITALANT RESEARCH INSTITUTE
  • Lisa Barcellos; University of California, Berkeley, School of Public Health
  • Eva Harris; University of California, Berkeley, School of Public Health
Preprint Dans Anglais | medRxiv | ID: ppmedrxiv-21266786
Journal article
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ABSTRACT
Serological surveillance studies of infectious diseases provide population-level estimates of infection and antibody prevalence, generating crucial insight into population-level immunity, risk factors leading to infection, and effectiveness of public health measures. These studies traditionally rely on detection of pathogen-specific antibodies in samples derived from venipuncture, an expensive and logistically challenging aspect of serological surveillance. During the COVID-19 pandemic, guidelines implemented to prevent the spread of SARS-CoV-2 infection made collection of venous blood logistically difficult at a time when SARS-CoV-2 serosurveillance was urgently needed. Dried blood spots (DBS) have generated interest as an alternative to venous blood for SARS-CoV-2 serological applications due to their stability, low cost, and ease of collection; DBS samples can be self-generated via fingerprick by community members and mailed at ambient temperatures. Here, we detail the development of four DBS-based SARS-CoV-2 serological methods and demonstrate their implementation in a large serological survey of community members from 12 cities in the East Bay region of the San Francisco metropolitan area using at- home DBS collection. We find that DBS perform similarly to plasma/serum in enzyme-linked immunosorbent assays and commercial SARS-CoV-2 serological assays. In addition, we show that DBS samples can reliably detect antibody responses months post-infection and track antibody kinetics after vaccination. Implementation of DBS enabled collection of valuable serological data from our study population to investigate changes in seroprevalence over an eight-month period. Our work makes a strong argument for the implementation of DBS in serological studies, not just for SARS-CoV-2, but any situation where phlebotomy is inaccessible.
Texte intégral: Disponible Collection: Preprints Base de données: medRxiv langue: Anglais Année: 2021 Type de document: Preprint
Texte intégral: Disponible Collection: Preprints Base de données: medRxiv langue: Anglais Année: 2021 Type de document: Preprint