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Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study.
Ren, Li-Li; Wang, Ye-Ming; Wu, Zhi-Qiang; Xiang, Zi-Chun; Guo, Li; Xu, Teng; Jiang, Yong-Zhong; Xiong, Yan; Li, Yong-Jun; Li, Xing-Wang; Li, Hui; Fan, Guo-Hui; Gu, Xiao-Ying; Xiao, Yan; Gao, Hong; Xu, Jiu-Yang; Yang, Fan; Wang, Xin-Ming; Wu, Chao; Chen, Lan; Liu, Yi-Wei; Liu, Bo; Yang, Jian; Wang, Xiao-Rui; Dong, Jie; Li, Li; Huang, Chao-Lin; Zhao, Jian-Ping; Hu, Yi; Cheng, Zhen-Shun; Liu, Lin-Lin; Qian, Zhao-Hui; Qin, Chuan; Jin, Qi; Cao, Bin; Wang, Jian-Wei.
  • Ren LL; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Wang YM; Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.
  • Wu ZQ; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100029, China.
  • Xiang ZC; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Guo L; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Xu T; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Jiang YZ; Vision Medicals Co., Ltd, Guangzhou, Guangdong 510000, China.
  • Xiong Y; Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei 430079, China.
  • Li YJ; Wuhan Center for Disease Control and Prevention, Wuhan, Hubei 430022, China.
  • Li XW; Vision Medicals Co., Ltd, Guangzhou, Guangdong 510000, China.
  • Li H; Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
  • Fan GH; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China.
  • Gu XY; Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.
  • Xiao Y; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100029, China.
  • Gao H; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China.
  • Xu JY; Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, China-Japan Friendship Hospital, Beijing 100029, China.
  • Yang F; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100029, China.
  • Wang XM; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China.
  • Wu C; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Chen L; Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
  • Liu YW; Department of Basic Medical Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
  • Liu B; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Yang J; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Wang XR; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Dong J; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Li L; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Huang CL; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Zhao JP; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Hu Y; Vision Medicals Co., Ltd, Guangzhou, Guangdong 510000, China.
  • Cheng ZS; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Liu LL; National Health Commission of the People's Republic of China Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
  • Qian ZH; Wuhan Jinyintan Hospital, Wuhan, Hubei 430022, China.
  • Qin C; Department of Pulmonary and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, Hubei 430030, China.
  • Jin Q; Department of Pulmonary and Critical Care Medicine, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, China.
  • Cao B; Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
  • Wang JW; Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei 430079, China.
Chin Med J (Engl) ; 133(9): 1015-1024, 2020 May 05.
Artigo em Inglês | MEDLINE | ID: covidwho-1722617
ABSTRACT

BACKGROUND:

Human infections with zoonotic coronaviruses (CoVs), including severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV, have raised great public health concern globally. Here, we report a novel bat-origin CoV causing severe and fatal pneumonia in humans.

METHODS:

We collected clinical data and bronchoalveolar lavage (BAL) specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital, Hubei province, China. Nucleic acids of the BAL were extracted and subjected to next-generation sequencing. Virus isolation was carried out, and maximum-likelihood phylogenetic trees were constructed.

RESULTS:

Five patients hospitalized from December 18 to December 29, 2019 presented with fever, cough, and dyspnea accompanied by complications of acute respiratory distress syndrome. Chest radiography revealed diffuse opacities and consolidation. One of these patients died. Sequence results revealed the presence of a previously unknown ß-CoV strain in all five patients, with 99.8% to 99.9% nucleotide identities among the isolates. These isolates showed 79.0% nucleotide identity with the sequence of SARS-CoV (GenBank NC_004718) and 51.8% identity with the sequence of MERS-CoV (GenBank NC_019843). The virus is phylogenetically closest to a bat SARS-like CoV (SL-ZC45, GenBank MG772933) with 87.6% to 87.7% nucleotide identity, but is in a separate clade. Moreover, these viruses have a single intact open reading frame gene 8, as a further indicator of bat-origin CoVs. However, the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV, indicating that these viruses might use the same receptor.

CONCLUSION:

A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans.
Assuntos

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Infecções por Coronavirus / Betacoronavirus Tipo de estudo: Estudo prognóstico Limite: Adulto / Idoso / Feminino / Humanos / Masculino / Meia-Idade Idioma: Inglês Revista: Chin Med J (Engl) Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: CM9.0000000000000722

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Infecções por Coronavirus / Betacoronavirus Tipo de estudo: Estudo prognóstico Limite: Adulto / Idoso / Feminino / Humanos / Masculino / Meia-Idade Idioma: Inglês Revista: Chin Med J (Engl) Ano de publicação: 2020 Tipo de documento: Artigo País de afiliação: CM9.0000000000000722