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Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents.
Haile, Simon; Nikiforuk, Aidan M; Pandoh, Pawan K; Twa, David D W; Smailus, Duane E; Nguyen, Jason; Pleasance, Stephen; Wong, Angus; Zhao, Yongjun; Eisler, Diane; Moksa, Michelle; Cao, Qi; Wong, Marcus; Su, Edmund; Krzywinski, Martin; Nelson, Jessica; Mungall, Andrew J; Tsang, Frankie; Prentice, Leah M; Jassem, Agatha; Manges, Amee R; Jones, Steven J M; Coope, Robin J; Prystajecky, Natalie; Marra, Marco A; Krajden, Mel; Hirst, Martin.
  • Haile S; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nikiforuk AM; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada.
  • Pandoh PK; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Twa DDW; BC Cancer Research Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Smailus DE; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nguyen J; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Pleasance S; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Wong A; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Zhao Y; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Eisler D; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Moksa M; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Cao Q; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Wong M; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Su E; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
  • Krzywinski M; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Nelson J; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Mungall AJ; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Tsang F; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Prentice LM; Provincial Laboratory Medicine Services, Provincial Health Services Authority, Vancouver, British Columbia, Canada.
  • Jassem A; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Manges AR; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada.
  • Jones SJM; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
  • Coope RJ; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada.
  • Prystajecky N; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Marra MA; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
  • Krajden M; British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Hirst M; Canada's Michael Smith Genome Sciences Centre, BC Cancer, Vancouver, British Columbia, Canada; Department of Microbiology and Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada. Electronic address: hirstm@mail.ubc.ca.
J Virol Methods ; 299: 114339, 2022 01.
Artigo em Inglês | MEDLINE | ID: covidwho-1472084
Preprint
Este artigo de periódico científico é provavelmente baseado em um preprint previamente disponível, por meio do reconhecimento de similaridade realizado por uma máquina. A confirmação humana ainda está pendente.
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ABSTRACT
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / COVID-19 Tipo de estudo: Estudo diagnóstico / Ensaios controlados aleatorizados Limite: Humanos Idioma: Inglês Revista: J Virol Methods Ano de publicação: 2022 Tipo de documento: Artigo País de afiliação: J.jviromet.2021.114339

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / COVID-19 Tipo de estudo: Estudo diagnóstico / Ensaios controlados aleatorizados Limite: Humanos Idioma: Inglês Revista: J Virol Methods Ano de publicação: 2022 Tipo de documento: Artigo País de afiliação: J.jviromet.2021.114339