Your browser doesn't support javascript.
SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data.
Nguyen, Dung; Simmonds, Peter; Steenhuis, Maurice; Wouters, Elise; Desmecht, Daniel; Garigliany, Mutien; Romano, Marta; Barbezange, Cyril; Maes, Piet; Van Holm, Bram; Mendoza, Joaquín; Oyonarte, Salvador; Fomsgaard, Anders; Lassaunière, Ria; Zusinaite, Eva; Resman Rus, Katarina; Avsic-Zupanc, Tatjana; Reimerink, Johan Hj; Brouwer, Fiona; Hoogerwerf, Marieke; Reusken, Chantal Bem; Grodeland, Gunnveig; Le Cam, Sophie; Gallian, Pierre; Amroun, Abdennour; Brisbarre, Nadège; Martinaud, Christophe; Leparc Goffart, Isabelle; Schrezenmeier, Hubert; Feys, Hendrik B; van der Schoot, C Ellen; Harvala, Heli.
  • Nguyen D; University of Oxford, Oxford, United Kingdom.
  • Simmonds P; University of Oxford, Oxford, United Kingdom.
  • Steenhuis M; Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Centre, Amsterdam, Netherlands.
  • Wouters E; Transfusion Research Centre, Belgian Red Cross-Flanders, Ghent, Belgium.
  • Desmecht D; Department of Pathology, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.
  • Garigliany M; Department of Pathology, Faculty of Veterinary Medicine, Liège University, Liège, Belgium.
  • Romano M; Immune Response service, Sciensano, Brussels, Belgium.
  • Barbezange C; National Influenza Centre, Sciensano, Brussels, Belgium.
  • Maes P; KU Leuven, Rega Institute, Clinical and Epidemiological Virology, Leuven, Belgium.
  • Van Holm B; KU Leuven, Rega Institute, Clinical and Epidemiological Virology, Leuven, Belgium.
  • Mendoza J; Vircell SL, Granada, Spain.
  • Oyonarte S; Andalusian Network of Transfusion Medicine, Tissues and Cells, Sevilla, Spain.
  • Fomsgaard A; Virus and Microbiological Special Diagnostics, Statens Serum Institute, Copenhagen, Denmark.
  • Lassaunière R; Virus and Microbiological Special Diagnostics, Statens Serum Institute, Copenhagen, Denmark.
  • Zusinaite E; Tartu University Institute of Technology, Tartu, Estonia.
  • Resman Rus K; Institute of Microbiology and Immunology, Ljubljana, Slovenia.
  • Avsic-Zupanc T; Institute of Microbiology and Immunology, Ljubljana, Slovenia.
  • Reimerink JH; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML, Bilthoven, the Netherlands.
  • Brouwer F; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML, Bilthoven, the Netherlands.
  • Hoogerwerf M; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML, Bilthoven, the Netherlands.
  • Reusken CB; Centre for Infectious Disease Control, WHO COVID-19 Reference Laboratory, RIVML, Bilthoven, the Netherlands.
  • Grodeland G; Dep. of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Le Cam S; Etablissement Français du Sang, La Plaine Saint Denis, France.
  • Gallian P; Etablissement Français du Sang, La Plaine Saint Denis, France.
  • Amroun A; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU Méditerranée Infection), Marseille, France.
  • Brisbarre N; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU Méditerranée Infection), Marseille, France.
  • Martinaud C; Unité des Virus Émergents (Aix-Marseille University - IRD 190 - Inserm 1207 - IHU Méditerranée Infection), Marseille, France.
  • Leparc Goffart I; Etablissement français du Sang Provence Alpes Côte d'Azur et Corse, Marseille, France.
  • Schrezenmeier H; Centre de Transfusion Sanguine des Armées, Clamart, France.
  • Feys HB; Unité de Virologie, Institut de Recherche Biomédicale des Armées, Marseille, France.
  • van der Schoot CE; Department of Transfusion Medicine, Ulm University, Ulm, Germany.
  • Harvala H; Institute for Clinical Transfusion Medicine and Immunogenetics, German Red Cross Blood Transfusion Service Baden-Wurttemberg - Hessen and University Hospital Ulm, Ulm, Germany.
Euro Surveill ; 26(27)2021 07.
文章 在 英语 | MEDLINE | ID: covidwho-1304570
ABSTRACT
We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca 10-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies.
主题 s
Keywords
全文
打印
XML
PubMed Links
Search on Google

全文: 可用 采集: 国际数据库 资料库: MEDLINE 主要主题: SARS-CoV-2 / COVID-19 研究类型: 随机对照试验 限制: 人类 国家/地区名称主题: 欧罗巴 语言: 英语 期刊主题: 传染病 年: 2021 类型: 文章 所属国家: 1560-7917.ES.2021.26.27.2100568

相关文档

MEDLINE
LILACS
LIS
全文
打印
XML
PubMed Links
Search on Google

全文: 可用 采集: 国际数据库 资料库: MEDLINE 主要主题: SARS-CoV-2 / COVID-19 研究类型: 随机对照试验 限制: 人类 国家/地区名称主题: 欧罗巴 语言: 英语 期刊主题: 传染病 年: 2021 类型: 文章 所属国家: 1560-7917.ES.2021.26.27.2100568