Epidermal growth factor and transforming growth factor alpha mRNA expression in human breast cancer bipsies : analysis in relation to estradiol, progesterone and EGF receptor content
Artagaveytia Cóppola, Nora; Le Penven, Salima; Falette, Nicole; Lucero Abreu, Rossana; Garófalo, Elsa; Saez, Simone.
J. Steroid Biochem. Molec. Biol
; 60(3-4): 221-28, 1997. ilus, tab
Artículo
en Inglés
| URUCAN | ID: bcc-1930
Among the peptide growth factors active in breast glandular cell proliferation epidermal growth factor (EGF) and transforming growth factor alpha (TGF x) are thought to play a major role in tumour development. They operate through binding to and activation of a common membrane receptor, defined as AGF-R. Their production is modulated by hormones and local growth factors. After it was shown by previous investigation in this laboratory that EGF-R could be detected in 90 percent of the tumours, but was masked by endogenous ligand in 36 percent of them, the question wa raised as to the level of the ligand's expression in tumour biopsies. Therefore, we investigated the expression of EGF and TGFx mRNA in 146 breast cancer biopsies by slot blot analysis using specific 32P- showed that EGF and TGFX coexisted in all tumour samples, and that their level of MRNA expression was similar in half of the tumours. Northern blot and polymerase chain reaction (PCR) analysis validated these findings. A significant direct correlation was found between the level of TGFx/EGF mRNA expression and the ER/progesterone receptor (PGR) content
Biblioteca responsable:
UY78.1
Ubicación: CDIC/BN-0536
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