Expression, purification and biological effect of a novel single chain Fv antibody and protamine fusion protein for the targeted delivery of siRNAs to FGFR3 positive cancer cells
In this study, a novel fusion protein R3P, which consists of FGFR3-ScFv and protamine, was generated in Escherichia coli by inclusion body expression strategy and Ni-NTA chromatography. Its yield reached 10 mg per liter of bacterial culture and its purity was shown to be higher than 95%. 1 µg of R3P could efficiently bind to about 2.5 pmol siRNAs and deliver siRNAs into FGFR3 positive RT112 and K562 cells. Annexin Vstaining results showed that R3P can deliver the amplified breast cancer 1 (AIB1) siRNAs to induce RT112 cellapoptosis.
Conclusion:
These results indicated that R3P was a promising carrier tool to deliver siRNAs into FGFR3 positive cancercells and to exert anti-tumor effect.