Abstract INTRODUCTION This study proposes to identify the
Leishmania species found in the
skin lesions of
cutaneous leishmaniasis (CL)
patients from Brasiléia municipality (Acre).
METHODS Skin biopsy imprints or
biopsy fragments were assayed via
kDNA-
PCR/
RFLP and FRET-
real-time PCR. RESULTS Of individuals with suspected CL, 18 were positive for
Leishmania kDNA.
Leishmania (Viannia) braziliensis (61.1%) and
Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples. CONCLUSIONS These results are congruent with the previous
reports in Acre and
Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the
epidemiology of the
disease in the
Amazon Basin.