Physicochemical characterization and evaluation of in vitro and in vivo toxicity of goldenberry extract nanoemulsion / Caracterização físico-química e avaliação da toxicidade in vitro e in vivo de nanoemulsão contendo extrato de goldenberry
Silva, Suelen Santos da; Bazana, Maiara Taís; Deus, Cassandra de; Machado, Marina Lopes; Cordeiro, Larissa Marafiga; Soares, Félix Alexandre Antunes; Libreloto, Daniele Rubert Nogueira; Rolim, Clarice Madalena Bueno; Menezes, Cristiano Ragagnin de; Codevilla, Cristiane Franco.
Ciênc. rural (Online)
; 49(8): e20190015, 2019. tab, graf
Artículo
en Inglés
| LILACS | ID: biblio-1045418
ABSTRACT Oil-in-water (O/W) nanoemulsion containing goldenberry extract was elaborated using a high-energy ultrasonic bath method. Physicochemical characterization of the formulation was carried out by determining pH, mean droplet diameter, polydispersity index (PDI) and zeta potential. Nanoemulsion toxicity was assessed using in vitro assays with tumor and non-tumor cell lines, and in vivo using Caenorhabditis elegans. The pH of the nanoemulsion was 3.84, the mean droplet diameter was 268 ± 7 nm, PDI 0.113 and zeta potential -13.94 mV. Results of the cytotoxicity assays employing non-tumor cells indicated that the extract associated or not with nanoemulsion maintained cell viability at different concentrations tested. In the assays using tumor lineage, it is observed that the nanoemulsion containing the extract had higher antitumor activity than the free extract. As for the in vivo tests, there was no change in the survival rate of the worms.
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